β_(2)-肾上腺素受体色谱模型的建立及在止咳平喘药筛选中的应用  

The Establishment ofβ_(2)-adrenergic Receptor Chromatographic Model and Its Application of Antitussive and Antiasthmatic Drugs in Screening

作　　者：尚丛珊[1] 侯亚妮[1] Shang Congshan;Hou Yani(Xi'an Peihua University,Shaanxi,710025)

机构地区：[1]西安培华学院,陕西710025

出　　处：《当代化工研究》2021年第21期175-176,共2页Modern Chemical Research

基　　金：陕西省教育厅专项科研计划项目“超快亲和萃取法在药物与β_(2)-肾上腺素受体相互作用中的应用研究”(编号:19JK0636)。

摘　　要：随着现代医学的发展,肾上腺素受体(AR)作为G-蛋白偶联受体家族的重要一员,受到人们的广泛关注。肾上腺素受体在体内有多种类型,其中肺部β_(2)-AR可以使气道平滑肌放松,常作为止咳平喘药的靶受体。该实验通过建立β_(2)-肾上腺素受体色谱模型,论证了β_(2)-肾上腺素受体在中药活性成分筛选中的作用。结果表明,基因重组技术获得的β_(2)-AR纯度较高,该受体色谱模型生物活性、稳定性较好,在中药活性成分筛选中能够发挥相应的作用。With the development of modern medicine,adrenergic receptor(AR),as an important member of the G-protein coupled receptor family,has received extensive attention.There are many types of adrenergic receptors in the body,among whichβ_(2)-AR in the lungs can relax airway smooth muscles,and is often used as a target receptor for cough and asthma drugs.This experiment demonstrates the role ofβ_(2)-adrenergic receptor in the screening of active ingredients of traditional Chinese medicine by establishing a chromatographic model ofβ_(2)-adrenergic receptor.The results show that theβ_(2)-AR obtained by genetic recombination technology has high purity,and the receptor chromatography model has good biological activity and stability,and can play a corresponding role in the screening of active ingredients of traditional Chinese medicine.

关 键 词：止咳平喘药筛选 β_(2)-肾上腺素受体 色谱模型 

分 类 号：R277.5[医药卫生—中医学]