细胞外基质力学微环境与细胞间相互作用的机制与特征  

Mechanism and characteristics of mechanical microenvironment of extracellular matrix and intercellular interaction

作　　者：闵子洋 穆妮热·艾力 郑耘昊 曾幸芷 边楠雁 邓双珊 谢静[1] Min Ziyang;Munire·Aili;Zheng Yunhao;Zeng Xingzhi;Bian Nanyan;Deng Shuangshan;Xie Jing(State Key Laboratory of Oral Diseases,West China School of Stomatology,Sichuan University,Chengdu 610041,Sichuan Province,China)

机构地区：[1]四川大学华西口腔医学院,口腔疾病研究国家重点实验室,四川省成都市610041

出　　处：《中国组织工程研究》2022年第25期4034-4045,共12页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(81600840,81771047),项目负责人:谢静。

摘　　要：背景:细胞外基质力学微环境通过调控细胞黏附、迁移、增殖和分化等,在人体器官发育、生理功能维持和疾病发生等方面发挥了重要作用。在再生医学和干细胞疗法中,基质力学微环境能引导植入细胞的存活、生长、增殖与分化,对调控植入细胞的行为发挥了重要的作用,对组织再生和干细胞治疗的成功率也起到关键作用。目的:综述细胞外基质微环境的力学信号对细胞行为包括细胞骨架重建、迁移、增殖、分化和细胞间交流的影响,从中阐明现有的分子调控机制,以期为组织工程和干细胞疗法中细胞与其力学微环境相互作用的实践性转化提供理论支撑。方法:检索PubMed数据库、万方数据库、CNKI中国期刊全文数据库收录的相关文献。英文检索词为“cytoskeleton,cell spreading,cell migration,cell proliferation,cell differentiation,cell communication,mechanotransduction,stiffness of substrate,surface topography,extracellular matrix,matrix”,中文检索词为“细胞骨架,细胞扩展,细胞迁移,细胞增殖,细胞分化,细胞交流,机械传导,基质硬度,表面形貌,细胞外基质,基质”,最终纳入161篇文献进行归纳总结。结果与结论:细胞外基质各种力学信号,如材料界面硬度、拓扑结构和亲/疏水性等,从力学识别、力学-化学信号转导、信号通路级联、下游蛋白活化、转录启动到蛋白表达来调控细胞的扩展、增殖、迁移、分化、交流和其他各种特殊生理性质,这对组织工程中细胞的定向培养和临床医学中的细胞靶向治疗具有重要意义。BACKGROUND:Mechanical properties of cellular extracellular matrix play a key role in human organ development,physiological function maintenance and disease occurrence by guiding cell adhesion,migration,proliferation and differentiation.In regenerative medicine and stem cell therapy,mechanical factors in extracellular matrix can direct the survival,growth,proliferation and differentiation of the implanted cells,which dictates cell fate of the implanted cells and determines the success of tissue regeneration and stem cell therapy.OBJECTIVE:To review the effects of mechanical signals of extracellular matrix microenvironment on cell behavior,including cytoskeleton reconstruction,migration,proliferation,differentiation and intercellular communication,and elucidate the existing molecular regulation mechanism in order to provide theoretical support for the practical transformation of the interaction between cells and their mechanical microenvironment in tissue engineering and stem cell therapy.METHODS:The articles were searched on PubMed,CNKI,and Wanfang databases with the key words of“cytoskeleton,cell spreading,cell migration,cell proliferation,cell differentiation,cell communication,mechanotransduction,stiffness of substrate,surface topography,extracellular matrix,matrix”in Chinese and English,respectively.Finally,161 articles met the criteria for review.RESULTS AND CONCLUSION:Various mechanical signals of extracellular matrix,such as material interface stiffness,topology and hydrophilicity/hydrophobicity,regulate cell expansion,proliferation,migration,differentiation,communication and other special physiological properties from mechanical recognition,mechanochemical signal transduction,signal pathway cascade,downstream protein activation,and transcriptional initiation to protein expression.It is of great significance for directional culture of cells in tissue engineering and cell targeted therapy in clinical medicine.

关 键 词：干细胞 力学刺激 细胞迁移 细胞增殖 细胞分化 细胞交流 力学传导 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学]