蛋白聚糖对牙发育再生及干细胞稳态的调控  

Proteoglycans in tooth development and its role in regulating stem cell homeostasis

作　　者：陈嘉文 孙天语 刘鹏[4] 吴补领[2,5] 吴靖漪 Chen Jiawen;Sun Tianyu;Liu Peng;Wu Buling;Wu Jingyi(Department of Stomatology,Nanfang Hospital,Southern Medical University,Guangzhou 510515,Guangdong Province,China;School of Stomatology,Southern Medical University,Guangzhou 510515,Guangdong Province,China;Department of Periodontology,Stomatological Hospital,Southern Medical University,Guangzhou 510280,Guangdong Province,China;Zhujiang Hospital,Southern Medical University,Guangzhou 510282,Guangdong Province,China;Southern Medical University-Shenzhen Stomatology Hospital(Pingshan),Shenzhen 518118,Guangdong Province,China;Center of Implant Dentistry,Stomatological Hospital,Southern Medical University,Guangzhou 510280,Guangdong Province,China)

机构地区：[1]南方医科大学南方医院口腔科,广东省广州市510515 [2]南方医科大学口腔医学院,广东省广州市510515 [3]南方医科大学口腔医院牙周科,广东省广州市510280 [4]南方医科大学珠江医院,广东省广州市510282 [5]南方医科大学深圳口腔医院(坪山),广东省深圳市518118 [6]南方医科大学口腔医院种植中心,广东省广州市510280

出　　处：《中国组织工程研究》2022年第25期4064-4069,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(81900956),项目负责人:吴靖漪;南方医科大学口腔医院博士后专项基金(PY2018025),项目负责人:吴靖漪;南方医科大学科研启动项目(QD2018N030),项目负责人:吴靖漪。

摘　　要：背景:牙再生研究是目前国际上口腔领域的热点问题之一,该研究的发展建立在对牙胚发育机制深入了解的基础之上。以往研究集中对牙发育相关细胞内信号调控网络进行探索,但对细胞外调控分子的研究尚且匮乏。蛋白聚糖作为细胞外基质及干细胞微环境的重要组成部分,参与细胞内外的信号传导,介导干细胞自我更新/分化平衡的调节。目的:综述蛋白聚糖对牙发育再生及干细胞稳态的调控作用及相关机制的最新研究结果,并对其未来发展方向进行展望。方法:检索PubMed、Web of Science和CNKI数据库等收录的有关蛋白聚糖与间充质干细胞稳态调控及蛋白聚糖参与牙发育再生的文章,英文检索词为“proteoglycans,glycosaminoglycans,stem cells homeostasis,tooth development,tooth renewal,cell signalling,heparan sulfate,chondroitin sulfate”,中文检索词为“蛋白聚糖,糖胺聚糖链,干细胞稳态,牙发育,牙再生,信号通路,硫酸乙酰肝素,硫酸软骨素”。根据纳入与排除标准对所有文章进行初筛后,保留质量和相关性较高的文章进行综述。结果与结论:蛋白聚糖作为细胞外基质及干细胞微环境的重要组成成分,不仅参与牙胚的发育过程,还能够干预牙胚干细胞以及间充质干细胞的归宿。阐明蛋白聚糖在牙胚发育以及对干细胞的调控作用,建立健全牙发育相关的细胞内外调控信号网络,为生物学牙再生研究提供新的思路。BACKGROUND:Tooth regeneration is one of the hot issues in dentistry.The development of tooth regeneration is based on the in-depth understanding of the mechanism of tooth germ development.Previous studies have focused on the exploration of intracellular signaling regulatory networks related to tooth development,but studies on extracellular regulatory molecules have been scarce.As an important component of extracellular matrix and stem cell microenvironment,proteoglycans are involved in signal transduction and mediate the balance between of self-renewal and differentiation of stem cells.OBJECTIVE:To review the research progress on proteoglycans in tooth development and its roles in mediating stem cell homeostasis and the mechanisms involved,and to prospect its future research focus.METHODS:PubMed,Web of Science and CNKI databases were searched for the articles concerning proteoglycans in regulating stem cell self-renewal homeostasis and tooth development with the search terms of“proteoglycans,glycosaminoglycans,stem cell homeostasis,tooth development,tooth renewal,cell signaling,heparan sulfate,chondroitin sulfate”in English and Chinese.After primarily screening based on the inclusion and exclusion criteria,the articles with high relevance were included for review.RESULTS AND CONCLUSION:As an important component of extracellular matrix and stem cell microenvironment,proteoglycans are extensively involved in the development of tooth germ and affect the destination of dental stem cells and mesenchymal stem cells.Clarifying the mechanism of proteoglycan in tooth germ development will be beneficial to establishing and perfecting the intracellular and extracellular regulatory signal network related to tooth development and provide a new idea for the study of biological tooth regeneration.

关 键 词：蛋白聚糖 糖胺聚糖链 干细胞稳态 牙发育 牙再生 信号通路 硫酸乙酰肝素 硫酸软骨素 综述 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学]