过量表达大白菜BrHSF16基因提高转基因拟南芥耐热性  

Overexpression of BrHSF16 gene in Chinese cabbage improves heat tolerance of transgenic Arabidopsis

作　　者：张慧敏 赵乾宇 李红颖 卢玲 张一卉[2] 邱念伟[4] 王凤德[2] 高建伟[2] ZHANG Huimin;ZHAO Qianyu;LI Hongying;LU Ling;ZHANG Yihui;QIU Nianwei;WANG Fengde;GAO Jianwei(College of Life Sciences,Shandong Normal University,Jinan 250014,China;Shandong Branch of National Vegetable Improvement Center,Shandong Key Laboratory of Greenhouse Vegetable Biology,Institute of Vegetables,Shandong Academy of Agricultural Sciences,Jinan 250100,China;Agricultural and Sideline Fishery Development Center of Qihe County,Qihe,Shandong 251100,China;College of Life Sciences,Qufu Normal University,Qufu,Shandong 273165,China)

机构地区：[1]山东师范大学生命科学学院,济南250014 [2]山东省农业科学院蔬菜研究所,山东省设施蔬菜生物学重点实验室,国家蔬菜改良中心山东分中心,济南250100 [3]齐河县农副渔业发展中心,山东齐河251100 [4]曲阜师范大学生命科学学院,山东曲阜273165

出　　处：《植物生理学报》2021年第10期1915-1925,共11页Plant Physiology Journal

基　　金：山东省泰山学者系列工程(tsqn201909167);山东省重点研发计划(2019GHZ014);山东省农业良种工程(2019LZGC0060101);山东省“渤海粮仓”科技示范工程(2019BHLC005);山东省农业科学院农业科技创新工程(CXGC2021A10);山东省自然科学基金(ZR2020MC144)。

摘　　要：热激转录因子(heat shock transcription factors,HSFs)是植物抵抗高温胁迫的重要调节因子。本研究利用RT-PCR的方法从大白菜中克隆了一个受热胁迫诱导显著上调的热激转录因子基因BrHSF16。该基因的开放阅读框(ORF)全长810 bp,预测编码269个氨基酸。系统进化分析表明,BrHSF16与AtHSFA7a处于同一分支,并且在氨基酸序列一致性上高达74.91%,从而暗示二者在功能上也具有相似性。进一步的研究发现,在拟南芥中过量表达BrHSF16基因可明显提高转基因植株在热胁迫下的生长状态和存活率。另外,过量表达BrHSF16基因后可促进AtHSFA2及其靶基因AtAPX2、AtHSP17.7、AtGolS1、AtHSP25.3和AtHSP70的转录表达。综上所述,BrHSF16可能作为正调控因子参与对热胁迫的响应。Heat shock transcription factors(HSFs) are important regulators of plant resistance to high temperature stress. In this study, an HSF gene BrHSF16 was cloned from Chinese cabbage by RT-PCR, which was significantly up-regulated under heat stress. Sequence analysis showed that the open reading frame(ORF) of BrHSF16 gene was 810 bp, encoding 269 amino acids. Phylogenetic analysis showed that BrHSF16 and AtHSFA7 a were in the same branch, and the amino acid sequence identity was as high as74.91%, suggesting that they also had functional similarity. Further studies showed that overexpression of Br HSF16 gene in Arabidopsis could significantly improve the growth status and survival rate of transgenic plants under heat stress. In addition, our study also found that overexpression of BrHSF16 gene can promote the transcriptional expression levels of AtHSFA2 and its target genes, including AtAPX2, AtHSP17.7, AtGolS1, AtHSP25.3 and AtHSP70. In summary, BrHSF16 may be a positive regulator involved in the response to heat stress.

关 键 词：大白菜 热胁迫 BrHSF16 基因克隆 功能分析 

分 类 号：S634.1[农业科学—蔬菜学]