乳源性复合益生菌促进db/db糖尿病小鼠白色脂肪棕色化的研究  

Lactogenic composite probiotics in promoting white fat browning in db/db diabetic mice

作　　者：哈普拉·托留汗 妮尕热·阿布都外力 木拉提艾则·玉素甫 新华·那比[1] Hapula TOLIUHAN;Nigare ABDUWELI;Murat Ezi YUSUP;Xinhua NABI(Department of Pharmacology,Xinjiang Medical University,Xinjiang 830011,China;不详)

机构地区：[1]新疆医科大学药理教研室,新疆乌鲁木齐830011

出　　处：《中国微生态学杂志》2021年第9期1000-1004,1008,共6页Chinese Journal of Microecology

基　　金：自治区高校科研计划自然科学项目(XJEDU2020I012)。

摘　　要：目的研究乳源性复合益生菌对db/db糖尿病小鼠白色脂肪棕色化细胞因子解偶联蛋白1(UCP1)、过氧化物酶体增殖物激活受体γ共激活因子1α(PGC1α)、R结构域蛋白16(PRDM16)表达的影响。方法将6周龄的SPF级db/db糖尿病雄性小鼠适应性喂养1周,随机分为糖尿病模型组、罗格列酮组及复合益生菌高剂量组和低剂量组,SC57BL/Ks雄性小鼠为正常对照组,每组8只。血糖仪检测不同时段空腹血糖(FBG)水平,ELISA法检测糖化血红蛋(HbA1c)含量,取各组小鼠皮下白色脂肪组织,HE染色观察脂肪组织形态,用Real time-PCR检测各组白色脂肪组织中UCP1、PGC1α、PRDM16 mRNA表达水平以及Western Blot检测各组脂肪组织中UCP1的表达。结果与模型组相比,复合益生菌组FBG、HbA1c水平明显下降,并且复合益生菌能够明显增加脂肪组织多室脂肪细胞数量,具有棕色化的趋向,并能够显著提高UCP1、PGC1α、PRDM16的mRNA表达和UCP1表达量。结论本研究发现乳源性复合益生菌能够促进白色脂肪棕色化从而改善胰岛素抵抗。Objective To explore the effects of lactogenic composite probiotics(LCP) on the expression of white adipose browning cytokine uncoupling protein 1(UCP1), peroxisome proliferator-activated receptor γ coactivator 1α(PGC1α) and PR domain-containing 16(PRDM16) in db/db diabetic mice. Methods Six-week-old SPF-grade db/db diabetic male mice were adaptively fed for one week and randomly divided into diabetes model group, rosiglitazone group, LCP high-dose group or LCP low-dose group, with SC57 BL/Ks as the normal control group(n=8). Fasting blood glucose(FBG) at different period of time was detected with blood glucose meter, and HbA1 c content was detected using ELISA. Subcutaneous white adipose fat was taken from each group, and the adipose tissue morphology was observed using HE staining. The mRNA expression of UCP1, PGC1α and PRDM16 were assayed with Q-PCR, and the expression of UCP1 protein was detected using Western Blot. Results Compared with the model group, the FBG and HbA1 c in the composite probiotic groups significantly decreased. The composite probiotics significantly increased the number of multilamellar adipocytes in adipose tissue, had a tendency to browning, and significantly increased the expression of UCP1, PGC1α and PRDM16 mRNAs and the expression of UCP1 protein. Conclusion Milk-derived composite probiotics can promote the browning of white adipose to improve insulin resistance.

关 键 词：DB/DB糖尿病小鼠 解偶联蛋白1 白色脂肪棕色化 乳源性复合益生菌 

分 类 号：R378[医药卫生—病原生物学]