HOXB5调控细胞周期蛋白参与小鼠肝脏再生  

HOXB5 participates in liver regeneration through regulating cyclins in mice

作　　者：杨凡 李海建 邱玉刚[1] 赵春贞 YANG Fan;LI Haijian;QIU Yugang;ZHAO Chunzhen(School of Public Health,Weifang Medical University,Weifang 261053,China;School of Pharmacy,Weifang Medical University)

机构地区：[1]潍坊医学院公共卫生学院,山东潍坊261053 [2]潍坊医学院药学院

出　　处：《潍坊医学院学报》2021年第5期330-333,共4页Acta Academiae Medicinae Weifang

基　　金：山东省高等学校科技计划项目(项目编号:J17KA256,J18KA280);山东省中医药科技发展计划项目(项目编号:2017-207)。

摘　　要：目的探讨HOXB5在小鼠肝脏再生过程中的作用及机制。方法HOXB5F/F小鼠和Alb-Cre小鼠交配获得HOXB5肝脏特异性敲除小鼠(KO小鼠)和同窝对照组小鼠(WT小鼠),收集70%肝脏切除手术后24,36,48和72h时间点的小鼠肝脏组织,并通过Western blotting和免疫组化染色等方法检测。结果70%肝脏切除手术后,KO小鼠在36,48h发现PCNA表达量明显高于对照组WT小鼠,同时Ki67和BrdU免疫组化染色也在KO小鼠中明显升高。经过RNA-sec测序结果提示,细胞周期基因在KO小鼠中有显著性改变。Western blotting结果提示KO小鼠肝脏Cyclin A2和Cyclin B1在48h表达明显升高。结论HOXB5通过调控Cyclin A2和Cyclin B1参与小鼠肝脏再生。Objective To explore the role and mechanism of HOXB5 during liver regeneration in mice.Methods Liver specific HOXB5 knockout mice(KO)and control mice(WT)were obtained by crossing of HOXB5F/F mice to Alb-Cre mice.Collecting the liver tissues at 24,36,48,and 72 hours after partial hepatectomy.Meanwhile,the collected samples were detected by Western blotting and immunohistochemical staining.Results The expression of PCNA in KO mice was significantly higher than that in WT mice at 36 and 48 hours after partial hepatectomy.In addition,Ki67 and BrdU immunohistochemical staining were also significantly increased in KO mice.The results of RNA-Sec sequencing indicated that the cell cycle gene was significantly altered in KO mice.Western blotting results showed that the expression of Cyclin A2 and Cyclin B1 in KO mice liver increased significantly after 48 hours.Conclusion HOXB5 is involved in mouse liver regeneration by regulating Cyclin A2 and Cyclin B1.

关 键 词：肝脏再生 HOXB5 Cyclin A2 Cyclin B1 

分 类 号：R318[医药卫生—生物医学工程]