BTH对‘怀黄’菊黑斑病抗性的影响  

Effect of BTH on resistance to black spot disease in Chrysanthemum morifolium ’Huaihuang’

作　　者：王丹丹 王跃武 解静 刘柯 杨军 王晓萌 李玮 赵喜亭[1,2] WANG Dandan;WANG Yaowu;XIE Jing;LIU Ke;YANG Jun;WANG Xiaomeng;LI Wei;ZHAO Xiting(College of Life Sciences,Henan Normal University,Xinxiang,Henan 453007,China;Engineering Technology Research Center of Nursing and Utilizati on of Genuine Chinese Crude Drugs of Henan Province,Xinxiang,Henan 453007,China;Botanical Garden of Xinxiang,Xinxiang,Henan 453000,China)

机构地区：[1]河南师范大学生命科学学院,河南新乡453007 [2]河南省道地药材保育及利用工程技术研究中心,河南新乡453007 [3]新乡市植物园,河南新乡453000

出　　处：《植物生理学报》2021年第9期1727-1736,共10页Plant Physiology Journal

基　　金：国家自然科学基金(U1704120和31372105)。

摘　　要：为了寻找安全有效的‘怀黄’菊(Chrysanthemum morifolium‘Huaihuang’)抗黑斑病的方法和途径,研究了苯并噻二唑(benzothiadiazole,BTH)对‘怀黄’菊黑斑病病原菌(Alternaria sp.)的诱抗效果,并对抗性相关指标进行了研究。结果显示,BTH可作为‘怀黄’菊抗黑斑病的诱抗剂,其最佳诱导浓度为0.5 mmol·L-1,诱导期为5 d,其诱抗效果可达61.40%~81.30%。BTH处理诱导了接种黑斑病病原菌后的‘怀黄菊’叶片超氧化物歧化酶(SOD)、过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)、谷胱甘肽还原酶(GR)等抗氧化酶和苯丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)、几丁质酶(CHT)、β-1,3-葡聚糖酶(GLU)等防御酶的活性提高,并且与不经BTH处理接种黑斑病病原菌相比,BTH+接种黑斑病病原菌处理下各防御酶活性的提高均达到显著性水平。这说明BTH诱导‘怀黄’菊抗黑斑病可能与其诱导提高‘怀黄’菊叶片中抗氧化酶及防御酶活性,尤其是防御酶的活性有关。In order to find a safe and effective way to resist black spot disease of Chrysanthemum morifolium ’Huaihuang’, the inducing resistance efficiency of benzothiadiazole(BTH) on Alternaria sp.(A. sp.) was studied, and the resistance-related indicators were also studied. The results showed that BTH could induce the resistance of ’Huaihuang’ against A. sp., the optimal concentration of BTH was 0.5 mmol·L–1 and the induction period was 5 days, and its inducing efficiency could reach 61.40%–81.30%. Besides, BTH treatment induced an increase in the activities of antioxidant enzymes(SOD, POD, APX, and GR) and defense enzymes(PAL, PPO, CHT, and GLU) in ’Huaihuang’ leaves after inoculation with A. sp.(BTH+A). Moreover, the increase in the activities of each defense enzyme reached significance when compared with inoculation A. sp. without BTH treatment(CK+A). These results suggested that the induced resistance to A. sp. in leaves of ’Huaihuang’ by BTH was likely to be related to the increased activities of antioxidant enzymes and defense enzymes, especially the activities of defense enzymes.

关 键 词：‘怀黄’菊 BTH 黑斑病 抗氧化酶 防御酶 

分 类 号：S435.672[农业科学—农业昆虫与害虫防治]