The blue light receptor CRY1 interacts with GID1 and DELLA proteins to repress GA signaling during photomorphogenesis in Arabidopsis  

作　　者：Ming Zhong Bingjie Zeng Dongying Tang Jiaxin Yang Lina Qu Jindong Yan Xiaochuan Wang Xin Li Xuanming Liu Xiaoying Zhao 

机构地区：[1]College of Biology,Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation,Hunan Hybrid Rape Engineering and Technology Research Center,Hunan University,Changsha 410082,China [2]Shenzhen Institute,Hunan University,Shenzhen 518057,China

出　　处：《Molecular Plant》2021年第8期1328-1342,共15页分子植物（英文版）

基　　金：supported by the National Natural Science Foundation of China(no.31171176);the Natural Science Foundation of Hunan Province(no.2020JJ4183);the Basic Research Program of Changsha Municipal Science and Technology(no.kq1901028).

摘　　要：Light is a critical environmental cue that regulates a variety of diverse plant developmental processes.Cryptochrome 1(CRY1)is the major photoreceptor that mediates blue light-dependent photomorphogenic responses such as the inhibition of hypocotyl elongation.Gibberellin(GA)participates in the repression of photomorphogenesis and promotes hypocotyl elongation.However,the antagonistic interaction between blue light and GA is not well understood.Here,we report that blue light represses GA-induced degradation of the DELLA proteins(DELLAs),which are key negative regulators in the GA signaling pathway,via CRY1,thereby inhibiting the GA response during hypocotyl elongation.Both in vitro and in vivo biochemical analyses demonstrated that CRY1 physically interacts with GA receptors-GA-INSENSITIVE DWARF 1 proteins(GID1s)-and DELLAs in a blue light-dependent manner.Furthermore,we showed that CRY1 inhibits the association between GID1s and DELLAs.Genetically,CRY1 antagonizes the function of GID1s to repress the expression of cell elongation-related genes and thus hypocotyl elongation.Taken together,our findings demonstrate that CRY1 coordinates blue light and GA signali ng for plant photomorphogenesis by stabilizing DELLAs through the binding and in activation of GID1s,providing new in sights into the mechanism by which blue light antagonizes the function of GA in photomorphogenesis.

关 键 词：cryptochrome 1 blue light gibberellic acid GID1 DELLA proteins 

分 类 号：Q94[生物学—植物学]