五味子乙素对铝中毒大鼠神经细胞凋亡及学习记忆能力的影响  

Effect of Sch B on neuronal apoptosis and the learning and memory ability of rats with aluminum intoxication

作　　者：程厚之 张丽凤 梁祚仁[1] 廖素婵 李艳丽 莫颂轶[1] 黄彦峰[1] 余双全 黄俊杰[1] Cheng Houzhi;Zhang Lifeng;Liang Zuoren;Liao Suchan;Li Yanli;Mo Songyi;Huang Yanfeng;Yu Shuangquan;Huang Junjie(School of Basic Medicine,Youjiang Medical University for Nationalities,Baise 533000,Guangxi,China)

机构地区：[1]右江民族医学院基础医学院,广西百色533000

出　　处：《右江民族医学院学报》2021年第5期588-591,共4页Journal of Youjiang Medical University for Nationalities

基　　金：广西自然科学基金项目(2018GXNSFAA281142);右江民族医学院高层次人才科研课题(yy2020gcky036)。

摘　　要：目的研究五味子乙素对慢性铝中毒大鼠神经细胞凋亡及学习记忆能力的影响。方法用AlCl 3连续喂养大鼠建立慢性铝中毒大鼠模型,用五味子乙素对铝中毒大鼠进行灌胃治疗30 d后,Morris水迷宫检测大鼠的学习记忆能力,记录空间探索实验潜伏期和平台穿越次数,原位细胞凋亡检测法(TUNEL)检测大鼠大脑皮层和海马神经细胞凋亡。结果五味子乙素高剂量组大鼠空间探索实验潜伏期比模型组的缩短,平台穿越次数比模型组的增多,差异有统计学意义(P<0.05)。铝中毒模型组大脑皮层和海马神经细胞凋亡率比空白对照组均明显增多(P<0.01);五味子乙素中、高剂量组大脑皮层和海马神经细胞凋亡率比模型组均明显减少(P<0.01)。结论五味子乙素提高铝中毒大鼠学习记忆能力与降低大脑皮层和海马神经细胞凋亡率有关。Objective To study the effect of Schisandrin B(Sch B)on neuronal apoptosis and the learning and memory ability of rats with chronic aluminum intoxication.Methods Rats were continuously fed with AlCl 3 to establish the rat models of chronic aluminum intoxication.After 30 days of intragastric treatment with Sch B,the rats were detected by Morris water maze for their learning and memory ability.The incubation period of spacial navigation testing and the times of crossing the testing platform were recorded.Terminal deoxynucleotidy transferase-mediated dUTP-biotin nick-end labeling(TUNEL)was used to detect the neuronal apoptosis in the cerebral cortex and hippocampus of rats.Results The incubation period of spacial navigation testing in the high-dose Sch B group was shorter than that of the model group,while the number of times of platform crossing were more than that of the model group,indicating significant differences(P<0.05).The model group had higher apoptosis rate of neurons in the cerebral cortex and hippocampus of rats than the blank control group(P<0.01).The medium-dose Sch B group and the high-dose Sch B group both had significantly lower apoptosis rate of neurons in the cerebral cortex and hippocampus of rats than the model group(P<0.01).Conclusion The effect of Sch B on the learning and memory ability of rats with aluminum intoxication was related to the decrease in neuronal apoptosis rate of cerebral cortex and hippocampus.

关 键 词：五味子乙素 慢性铝中毒 神经细胞凋亡 大脑皮层 海马 学习记忆 

分 类 号：R33[医药卫生—人体生理学] R285.5[医药卫生—基础医学]