热应激联合HBsAg刺激增强人树突状细胞免疫学特性  

Heat stress combined with HBsAg stimulation to enhance the immunological characteristics of human dendritic cells

作　　者：黄新亮 余方流 王凤各 董博翰 Huang Xinliang;Yu Fangliu;Wang Fengge;Dong Bohan(School of Basic Medicine,Wannan Medical College,Wuhu 241002,Anhui,China)

机构地区：[1]皖南医学院基础医学院,安徽芜湖241002

出　　处：《右江民族医学院学报》2021年第5期632-635,共4页Journal of Youjiang Medical University for Nationalities

基　　金：安徽高校省级自然科学研究重点项目(KJ2019A0415);高校优秀青年人才支持计划(gxyqZD2019040);皖南医学院第三批学术和技术带头人及后备人选资助计划(校政2019第81号)。

摘　　要：目的探索热应激联合乙肝病毒表面抗原(HBsAg)刺激的树突状细胞(DC)的免疫特性及其诱导的特异性细胞毒性T细胞(CTL)的细胞毒效应。方法选择28例健康志愿者分为4组,每组7例,热应激联合HBsAg组用人外周血单个核细胞制备DC,DC负载HBsAg后在42℃下持续放置30 min,检测DC表面分子。ELISA法检测HSP90,再与自身淋巴细胞共培养7 d,用流式细胞仪检测CD3+CD8+T数量。用LDH法检测特异性CTL细胞毒效应,与热应激联合HBsAg组不同的是热应激组DC不负载HBsAg、HBsAg组不用42℃温度处理、对照组既不负载HBsAg也不经42℃温度处理。结果热应激联合HBsAg组DC中表达CD80、CD40、HLA-DR分子的比率、HSP90水平、T细胞中CD3+CD8+T的比率及CTL细胞毒效应明显高于热应激组、HBsAg组和对照组(P<0.05);且热应激联合HBsAg组HSP90水平与DC表达CD80、CD40及HLA-DR的比率呈正相关(r分别为0.73、0.69、0.78)。结论与热应激、HBsAg分别刺激DC比较,热应激联合HBsAg刺激的DC提呈抗原的活性增强,且能诱导更明显的特异性CTL细胞毒效应。Objective To investigate the immune characteristics of dendrite cells(DC)stimulated by heat stress combined with hepatitis B surface antigen(HBsAg)and explore the cytotoxic effect of specific cytotoxic T lymphocyte(CTL)induced by them.Methods Twenty-eight healthy volunteers were selected and divided into 4 groups with 7 cases in each group.In the group of heat stress combining with HBsAg,DCs were prepared by using human peripheral blood mononuclear cells(PBMC).The surface molecules of DCs were detected after these DCs were loaded with HBsAg and placed at 42℃for 30 min.The HSP90 was detected by ELISA.Then the DCs were co-cultured with autologous lymphocytes for 7 days and the number of CD3+CD8+T was detected by flow cytometry after the co-culture.The cytotoxic effect of specific CTL was detected by LDH(lumbar disc herniation).Different from those of the group of heat stress combining with HBsAg,DCs in the heat stress group were not loaded with HBsAg;those in the HBsAg group were not treated at 42℃,and those in the control group were neither loaded with HBsAg nor treated at 42℃.Results The group of heat stress combining with HBsAg had significantly higher ratios of CD80,CD40 and HLA-DR expressed in DCs,higher HSP90 level,and higher ratio of CD3+CD8+T in T cells and stronger cytotoxic effect of CTL than the heat stress group,the HBsAg group as well as the control group(P<0.05).And the HSP90 level of the group of heat stress combing with HBsAg was positively correlated with the ratios of CD80,CD40 and HLA-DR expressed in DCs(r was 0.73,0.69 and 0.78,respectively).Conclusion Compared with the DCs stimulated by heat stress and HBsAg respectively,the DCs stimulated by heat stress combined with HBsAg have enhanced antigen activity and induce more obvious cytotoxic effect of specific CTL.

关 键 词：热应激 乙型肝炎病毒表面抗原 树突细胞 

分 类 号：R392.11[医药卫生—免疫学]