电针干预面神经压榨模型大鼠促进面神经的再生  

Electroacupuncture promotes facial nerve regeneration in a rat model of facial nerve crush

作　　者：费静 陶美惠 李雷激 Fei Jing;Tao Meihui;Li Leiji(Department of Otorhinolaryngology,Head and Neck Surgery,The Affiliated Hospital of Southwest Medical University,Luzhou 646000,Sichuan Province,China;Department of Otolaryngology,Chengdu Shuangliu District First People’s Hospital,Chengdu 610200,Sichuan Province,China)

机构地区：[1]西南医科大学附属医院耳鼻咽喉头颈外科,四川省泸州市646000 [2]成都市双流区第一人民医院耳鼻咽喉科,四川省成都市610200

出　　处：《中国组织工程研究》2022年第11期1728-1733,共6页Chinese Journal of Tissue Engineering Research

基　　金：泸州-西南医大合作项目基金资助(2017LZXNYD-J34),项目负责人:李雷激。

摘　　要：背景:电针可促进面神经再生,但其机制不明。血管内皮生长因子是一类可促进血管再生、增加血管通透性的细胞因子。目的:观察电针对SD大鼠面神经颊支压榨损伤所致周围性面瘫的治疗效果,探究血管内皮生长因子/MAPK/ERK信号通路在上述过程中的作用。方法:56只SD大鼠随机分成正常组(8只)、模型组(24只)、电针组(24只)。模型组和电针组均制作右侧面神经颊支压榨损伤模型,电针组术后每日于右侧“颊车”“地仓”穴行电针治疗,术后4,14,28 d,记录各组大鼠面瘫症状并评分,观察面神经元的形态及尼氏体变化,血管内皮生长因子的表达部位、强度以及血管内皮生长因子、ERK、p-ERK蛋白在面神经元组织的表达水平。结果与结论:①模型组面瘫症状改善不如电针组明显,行为学评分在各时间点均低于电针组;②电针组面神经元尼氏体的形态恢复较模型组早且完全;③在术后各时间点电针组血管内皮生长因子的表达均高于模型组及正常组,p-ERK蛋白的表达均高于模型组,自术后14 d开始高于正常组;④结果表明,电针可上调血管内皮生长因子在面神经元中的表达,促进压榨损伤所致的周围性面瘫的恢复,其机制可能与激活MAPK/ERK信号通路,发挥面神经元的保护作用有关。BACKGROUND:The mechanism of electroacupuncture to promote facial nerve regeneration is unknown.Vascular endothelial growth factors(VEGF)are a type of cytokines that can promote revascularization and increase vascular permeability.OBJECTIVE:To observe the therapeutic effect of electroacupuncture on peripheral facial paralysis induced by crush injury of the buccal facial nerve in SpragueDawley rats,and to explore the role of VEGF/mitogen-activated protein kinase(MAPK)/extracellular signal-regulated kinase(ERK)signaling pathway in the above process.METHODS:Sprague-Dawley rats were randomly divided into a normal group(8 rats),a model group(24 rats)and an electroacupuncture group(24 rats).The facial nerve crush injury models were established in the model and the electroacupuncture groups.Rats in the electroacupuncture group then received electroacupuncture treatment at the right"Jiache"and"Dicang"acupoints every day after the operation.At 4,14,and 28 days after the operation,the symptoms of facial paralysis in the rats of each group were recorded and scored.The morphology and Nissl body changes of facial motoneurons were observed.The expression site and intensity of VEGF as well as the expression levels of VEGF,ERK,and p-ERK proteins in facial motoneurons were measured.RESULTS AND CONCLUSION:The improvement of facial paralysis in the model group was not as obvious as that in the electroacupuncture group,and the behavioral scores were lower than those in the electroacupuncture group at all time points.The morphological recovery of facial motoneurons and Nissl bodies in the electroacupuncture group was earlier and more complete than that in the model group.The protein expressions of VEGF at all time points in the electroacupuncture group were higher than those in the normal and model groups.The expression of p-ERK in the electroacupuncture group was higher than that in the model group,and began to be higher than that in the normal group at 14 days after operation.To conclude,electroacupuncture can up-regulate the expres

关 键 词：电针 面神经元 颊车 地仓 血管内皮生长因子 信号通路 大鼠 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学]