孵化前期二氧化碳调控对蛋鸡种蛋孵化胚胎的影响  

Effects of carbon dioxide regulation during early incubation stage on the hatching embryos of layer eggs

作　　者：童勤 朱丽蓉 刘畅 郑炜超[1,2,3] 韩胜强 李杜 Tong Qin;Zhu Lirong;Liu Chang;Zheng Weichao;Han Shengqiang;Li Du(College of Water Resources and Civil Engineering,China Agricultural University,Beijing 100083,China;Key Laboratory of Agricultural Engineering in Structure and Environment,Ministry of Agriculture and Rural Affairs,Beijing 100083,China;Beijing Engineering Research Center for Animal Healthy Environment,Beijing 100083,China;Jiangsu Lihua Animal Husbandry Co.,Ltd.,Changzhou 213168,China;Beijing Huadu Yukou Poultry Industry Co.,Ltd.,Beijing 101206,China)

机构地区：[1]中国农业大学水利与土木工程学院,北京100083 [2]农业农村部设施农业工程重点实验室,北京100083 [3]北京市畜禽健康养殖环境工程技术研究中心,北京100083 [4]江苏立华牧业股份有限公司,常州213168 [5]北京市华都峪口禽业有限责任公司,北京101206

出　　处：《农业工程学报》2021年第16期177-183,共7页Transactions of the Chinese Society of Agricultural Engineering

基　　金：国家自然科学基金(31802109)。

摘　　要：为探究孵化前期高浓度CO_(2)调控对蛋鸡种蛋孵化的影响机制,该研究以京红1号蛋鸡种蛋为试验对象,在孵化前期(0~10 d)处理组通过补充CO_(2)的方式保持CO_(2)浓度1%,对照组CO_(2)浓度小于0.25%,对比研究了1%CO_(2)浓度处理对种蛋孵化的影响。结果表明,CO_(2)处理组与对照组的受精蛋孵化率无显著性差异(P>0.05);第9天和第12天CO_(2)处理组的胚胎质量和相对胚胎质量显著高于对照组(P<0.05);第3天、第6天和第9天CO_(2)处理组的蛋白pH值显著低于对照组(P<0.05);第11天,CO_(2)处理组和对照组尿囊绒毛膜(CAM,Chorioallantoic Membrane)血管发育密度无显著性差异(P>0.05);第0天、第6天、第12天两组的蛋壳和胚胎钙含量不存在显著差异。在蛋鸡种蛋孵化前期(0~10 d)保持1%浓度的CO_(2),降低了蛋白pH值,加速了胚胎发育,但未影响孵化率。Environmental parameters generally determine the incubation performance and chick quality,one of which is carbon dioxide(CO_(2)).However,the mechanism still remains unclear,particularly the effects of high CO_(2)levels during early incubation on the hatching quality.In this study,three batches of fertile eggs from Jinghong No.1 were incubated in two small-scale incubators,in order to investigate the influence of normal and higher CO_(2)levels during the early stage of incubation(0-10 d).The treatment and control incubator maintained the CO_(2)concentration of 1%and below 0.25%,respectively.The incubators were also swapped for the next batch.A higher CO_(2)level was controlled during the incubation using a purpose-built system with CO_(2)sensors and a CO_(2)gas adding unit.The specific parameters were measured,including hatchability,chick quality,embryo weight,and relative embryo weight at the 6^(th),9^(th),12^(th),15^(th),and 18^(th) day,vascular development density of allantoic chorion(CAM)at day 11,albumen pH at day 0,day 3,day 6,day 9,day 12 and day 15,and the calcium content of eggshells and embryos at day 0,day 6 and day 12.The results showed that the CO_(2)concentrations in the treatment and control incubator were achieved the target levels of about(0.96±0.04)%,lower than 0.25%.There was no significant difference between the two groups in hatchability and fertilized egg hatchability,where were(89.17±2.53)%and(91.57±1.03)%in the treatment and control group,respectively.Moreover,the higher CO_(2)during early stage did not have much effect on the chick quality.The vascular development density of CAM on the 11th day had no significant difference between the treated and control groups,because the O2 concentration was maintained around(20.2±0.1)%.There was also no significant difference in the calcium content of eggshells and embryos in the treated and control groups(P>0.05),but the calcium content of embryos increased significantly from day 6 to day 12 in both groups(P<0.05).However,the embryo weight and rel

关 键 词：二氧化碳 动物 孵化 胚胎发育 孵化率 

分 类 号：S8[农业科学—畜牧兽医]