不同孔隙率多孔ZnO/羟基磷灰石复合材料的力学性能与生物相容性  

Mechanical properties and biocompatibility of porous ZnO/hydroxyapatite composites with different porosities

作　　者：孟增东[1] 朱斌 张亚楠 罗丽琳[3] 张玉勤[2] Meng Zengdong;Zhu Bin;Zhang Yanan;Luo Lilin;Zhang Yuqin(Department of Orthopedics,the First People’s Hospital of Yunnan Province,Kunming 650032,Yunnan Province,China;School of Materials Science and Engineering,Kunming University of Science and Technology,Kunming 650093,Yunnan Province,China;Department of Orthopedic Pathology,the First People’s Hospital of Yunnan Province,Kunming 650032,Yunnan Province,China)

机构地区：[1]云南省第一人民医院(昆明理工大学附属医院)骨科,云南省昆明市650032 [2]昆明理工大学材料科学与工程学院,云南省昆明市650093 [3]云南省第一人民医院(昆明理工大学附属医院)骨科病理科,云南省昆明市650032

出　　处：《中国组织工程研究》2022年第22期3498-3504,共7页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(31860264),项目负责人:孟增东;云南省应用基础研究重点项目(2019FA029),项目负责人:孟增东。

摘　　要：背景:前期研究发现,在纳米羟基磷灰石中加入氧化锌并将其多孔化可明显提高磷灰石的形成能力,同时具有良好的生物相容性,但孔隙率的提高会导致力学性能的劣化。目的:观察不同孔隙率下多孔氧化锌/羟基磷灰石复合材料微观结构、孔隙特征、力学性能、体外矿化与降解性能的变化,以及在适宜孔隙率下该复合材料的细胞相容性。方法:通过改变造孔剂(医用级碳酸氢铵)添加量来控制复合材料的孔隙率,利用放电等离子烧结技术制备不同孔隙率(42%,51%,62%)的多孔氧化锌/羟基磷灰石复合材料,研究3种复合材料的微观结构、孔隙特征、力学性能、体外矿化与降解性能。将兔骨髓间充质干细胞接种于适宜孔隙率的多孔氧化锌/羟基磷灰石复合材料表面,观察细胞黏附和增殖情况。结果与结论:(1)扫描电镜显示,随着孔隙率的提高,复合材料的孔隙数量明显增加,通孔结构增加,表面也出现微裂纹,复合材料的孔径在0-500μm之间;(2)随着孔隙率的提高,复合材料的抗压强度与弹性模量降低,抗压强度由148 MPa下降至56 MPa,弹性模量由6.5 GPa下降至3.5 GPa;(3)体外矿化与降解实验显示,随着孔隙率的提高,复合材料表面及孔隙结构中出现的类骨磷灰石沉积物增加,降解速率加快;(4)综合上述实验结果,选取孔隙率为42%的复合材料与兔骨髓间充质干细胞共培养,直接接触培养实验显示,细胞在复合材料表面与空隙内部生长黏附良好;复合材料浸提液CCK-8实验显示,随着培养时间的延长,兔骨髓间充质干细胞快速增殖;(5)结果表明,孔隙率为42%的多孔氧化锌/羟基磷灰石复合材料具有良好的力学性能、降解性能与生物相容性。BACKGROUND:Earlier studies have found that adding zinc oxide to nano-hydroxyapatite and making it porous can significantly improve the apatite-forming ability and simultaneously have good biocompatibility,but the increase in porosity will lead to the deterioration of mechanical properties.OBJECTIVE:To observe the changes in the microstructure,pore characteristics,mechanical properties,in vitro mineralization and degradation properties of porous zinc oxide/hydroxyapatite composites under different porosities,as well as the cell compatibility of the composites under suitable porosity.METHODS:The porosity of the composite material was controlled by changing the added amount of pore former(medical grade ammonium bicarbonate).Porous zinc oxide/hydroxyapatite composite materials with different porosities(42%,51%,62%)were prepared by spark plasma sintering technology to study the microstructure,pore characteristics,mechanical properties,in vitro mineralization and degradation properties of three kinds of composite materials.Rabbit bone marrow mesenchymal stem cells were inoculated on the surface of porous zinc oxide/hydroxyapatite composite material with appropriate porosity,and cell adhesion and proliferation were observed.RESULTS AND CONCLUSION:(1)Scanning electron microscopy showed that with the increase of porosity,the number of pores in the composite material increased significantly;the through-hole structure increased;and microcracks appeared on the surface.The pore size of the composite material was between 0-500μm.With the increase of porosity,compressive strength and elastic modulus of the composite were reduced;the compressive strength decreased from 148 MPa to 56 MPa;the elastic modulus decreased from 6.5 GPa to 3.5 GPa.(3)In vitro degradation and mineralization experiments showed that with improved composite surface porosity,bone apatite deposition species increased,and the degradation rate was accelerated.(4)Based on the above experimental results,the composite material with the porosity of 42%was selected

关 键 词：羟基磷灰石 多孔化 孔隙率 力学性能 磷灰石 降解行为 细胞黏附 细胞增殖 

分 类 号：R459.9[医药卫生—治疗学] R628[医药卫生—临床医学]