聚左旋乳酸己内酯/丝素蛋白小口径人工血管细胞共培养及体内生物相容性  

Cell co-culture and in vivo biocompatibility of poly(L-lactic caprolactone)/silk fibroin small-diameter artificial blood vessels

作　　者：刘月 蒋紫仪 李晶晶[2,3] 孟凯[1] 赵荟菁[1] Liu Yue;Jiang Ziyi;Li Jingjing;Meng Kai;Zhao Huijing(College of Textile and Clothing Engineering,Soochow University,Suzhou 215123,Jiangsu Province,China;Institute for Cardiovascular Science,Soochow University,Suzhou 215021,Jiangsu Province,China;Department of Cardiovascular Surgery,First Affiliated Hospital of Soochow University,Suzhou 215006,Jiangsu Province,China)

机构地区：[1]苏州大学纺织与服装工程学院,江苏省苏州市215123 [2]苏州大学心血管病研究所,江苏省苏州市215021 [3]苏州大学附属第一医院心脏大血管外科,江苏省苏州市215006

出　　处：《中国组织工程研究》2022年第22期3505-3513,共9页Chinese Journal of Tissue Engineering Research

基　　金：江苏省高等学校自然科学研究重大基金项目(19KJA610004),项目负责人:赵荟菁;中国纺织工业联合会科技指导性项目(2019021),项目负责人:赵荟菁。

摘　　要：背景:自体旁路移植是心血管疾病最佳的手术方案,但自体血管来源有限,远远不能满足巨大的临床需求,因此小口径人工血管的研发具有十分重要的意义,然而小口径人工血管由于易产生血栓和内膜增生等问题还没有真正应用于临床。目的:观察人脐静脉内皮细胞和血管平滑肌细胞在聚左旋乳酸己内酯/丝素蛋白电纺膜上的生长情况,以及聚左旋乳酸己内酯/丝素蛋白小口径人工血管在大鼠体内的通畅性、组织相容性和降解性能。方法:采用静电纺丝技术制备聚左旋乳酸己内酯/丝素蛋白小口径双层人工血管,加载血管内皮生长因子于人工血管上,最后将肝素加载至人工血管上。将人脐静脉内皮细胞和血管平滑肌细胞分别接种到加载与未加载血管内皮生长因子的人工血管上,通过激光共聚焦显微镜、扫描电镜和MTT法分析细胞在人工血管材料上的生长情况。以加载与未加载血管内皮生长因子的人工血管电纺膜代替细胞小室中的Transwell膜,将人脐静脉内皮细胞和血管平滑肌细胞共同接种至电纺膜上,通过激光共聚焦显微镜、扫描电镜和MTT法分析细胞在人工血管材料上的生长情况。将加载血管内皮生长因子与肝素的人工血管植入大鼠颈动脉,检测人工血管的通畅性与组织相容性、降解性。结果与结论:(1)细胞单独接种于人工血管上:激光共聚焦显微镜、扫描电镜与MTT分析显示,接种在加载血管内皮生长因子人工血管上的人脐静脉内皮细胞多于未加载血管内皮生长因子人工血管,加载与未加载血管内皮生长因子的血管平滑肌细胞数量基本相同。(2)两细胞共同接种于人工血管上:激光共聚焦显微镜、扫描电镜与MTT分析显示,加载与未加载血管内皮生长因子人工血管上的共培养细胞数量均多于对应人工血管上的单独细胞接种数量,但小于两种单种细胞数之和。(3)动物体�BACKGROUND:The autologous bypass grafting is the best surgical scheme.However,the source of autologous blood vessels is limited,which is far from meeting the huge clinical needs.Therefore,the research and development of small-diameter artificial blood vessels are of great significance.However,smalldiameter artificial blood vessels have not been truly applied in clinical practice due to the problems of easy thrombosis and intimal hyperplasia.OBJECTIVE:To explore the growth of human umbilical vein endothelial cells and vascular smooth muscle cells on poly(L-lactic caprolactone)/silk fibroin electrospun membrane,the patency,histocompatibility and degradability of poly(L-lactic caprolactone)/silk fibroin small-diameter artificial blood vessels in rats.METHODS:Electrospinning technology was used to prepare poly(L-lactic caprolactone)/silk fibroin small-caliber artificial blood vessels.Vascular endothelial growth factors were loaded on the artificial blood vessel,and heparin was finally loaded on the artificial blood vessel.Human umbilical vein endothelial cells and vascular smooth muscle cells were respectively seeded on artificial blood vessels loaded and unloaded with vascular endothelial growth factor.The growth of cells on artificial blood vessels was analyzed by confocal laser scanning microscope,scanning electron microscope,and MTT assay.Transwell membrane in cell chamber was replaced with poly(L-lactic caprolactone)/silk fibroin electrospun membrane.Human umbilical vein endothelial cells and vascular smooth muscle cells were inoculated on poly(L-lactic caprolactone)/silk fibroin material for cell co-culture.The growth of cells on poly(L-lactic caprolactone)/silk fibroin materials was analyzed by confocal laser scanning microscope,scanning electron microscope,and MTT assay.The artificial blood vessel loaded with vascular endothelial growth factor and heparin was implanted into the rat carotid artery.The patency,histocompatibility,and degradability of the artificial blood vessel were detected.RESULTS AND CONCLUSI

关 键 词：小口径人工血管 聚左旋乳酸己内酯 丝素蛋白 细胞共培养 体内实验 

分 类 号：R459.9[医药卫生—治疗学] R318.08[医药卫生—临床医学]