细菌纳米纤维素/聚多巴胺复合管作为小口径人工血管的潜力  

Potential of bacterial nanocellulose/polydopamine composite tubes as small-diameter artificial blood vessel

作　　者：刘亮 胡高铨 韦昭 陈琳[1,2,3] 洪枫 Liu Liang;Hu Gaoquan;Wei Zhao;Chen Lin;Hong Feng(College of Chemistry,Chemical Engineering and Biotechnology,Donghua University,Shanghai 201620,China;Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine,Donghua University,Shanghai 201620,China;Scientific Research Base of Bacterial Nanofiber Manufacturing and Composite Technology,China Textile Engineering Society,Shanghai 201620,China)

机构地区：[1]东华大学化学化工与生物工程学院,上海市201620 [2]上海纳米生物材料与再生医学工程技术研究中心,上海市201620 [3]中国纺织工程学会,细菌纳米纤维制造与复合技术科研基地,上海市201620

出　　处：《中国组织工程研究》2022年第22期3535-3542,共8页Chinese Journal of Tissue Engineering Research

基　　金：国家重点研发计划课题(2018YFC1105501),项目负责人:洪枫。

摘　　要：背景:临床应用上缺乏小口径(<6 mm)人工血管。目的:探究细菌纳米纤维素/聚多巴胺(bacterial nanocellulose/polydopamine,BNC/PDA)复合管用于小口径人工血管的潜力。方法:通过外硅胶管反应器制备细菌纳米纤维素小口径人工血管,将经纯化的纤维素管浸渍在不同质量浓度(0.1,0.5,1.0,1.5,2.0 g/L)的多巴胺溶液中进行自聚合反应,制备BNC/PDA复合管,表征细菌纳米纤维素管与BNC/PDA复合管的微观结构、红外光谱、密度、持水量、水渗透量、爆破和缝合强度、轴向力学性能及血液和细胞相容性等性质。结果与结论:(1)场发射扫描电镜显示,所有管的内表面是由纳米纤维搭建的3D网络结构,纤维分布均匀、结构致密,随着多巴胺质量浓度的增加,人工血管的纤维直径增大。(2)随着多巴胺质量浓度的增加,人工血管的密度、爆破和缝合强度及轴向力学性能增大,水渗透量和持水量减小。(3)溶血率与血小板黏附实验结果显示,细菌纳米纤维素管和BNC/PDA复合管的溶血率均为不溶血等级,复合管比细菌纳米纤维素管较少黏附血小板;全血凝固实验结果显示,复合管较细菌纳米纤维素管有较强的促凝血性能。(4)CCK-8实验结果显示,与单纯的细菌纳米纤维素相比,BNC/PDA-0.1复合管可促进人脐静脉内皮细胞的增殖,其余4种复合管抑制了细胞的增殖,并且BNC/PDA-1.5与BNC/PDA-2.0复合管表现出明显的细胞毒性;钙黄绿素荧光染色结果显示,细菌纳米纤维素管及BNC/PDA-0.1、BNC/PDA-0.5、BNC/PDA-1.0复合管表面的细胞可持续增殖,其中BNC/PDA-0.1复合管表面的细胞数量多于细菌纳米纤维素管。(5)结果表明,BNC/PDA复合管应用于小口径人工血管有一定的潜力,且后续可进一步接枝活性大分子实现功能化。BACKGROUND:There is a lack of small-caliber artificial blood vessels(<6 mm)in clinical application.OBJECTIVE:To explore the potential of bacterial nanocellulose/polydopamine(BNC/PDA)composite tubes for small-caliber artificial blood vessels.METHODS:Bacterial nanocellulose small-caliber artificial blood vessel was prepared through an external silicone tube reactor,and the purified cellulose tube was immersed in dopamine solutions of different mass concentrations(0.1,0.5,1.0,1.5,2.0 g/L)for self polymerization reaction.After preparation of BNC/PDA composite tubes,the microstructure,infrared spectrum,density,water holding capacity,water permeability,burst and suture strength,axial mechanical properties,and blood and cell compatibility of the bacterial nanocellulose tube and BNC/PDA composite tubes were characterized.RESULTS AND CONCLUSION:(1)The field emission scanning electron microscope showed that the inner surface of all tubes was a 3 D network structure built by nanofibers,with uniform fiber distribution and compact structure.With the increase of dopamine mass concentration,the fiber diameter of artificial blood vessels increased.(2)As the mass concentration of dopamine increased,the density,burst and suture strength and axial mechanical properties of artificial blood vessels increased,and the water penetration and water holding capacity decreased.(3)The hemolysis rate and platelet adhesion test results showed that the hemolysis rate of the bacterial nanocellulose tube and the BNC/PDA composite tubes were both non-hemolytic grades.The composite tube adhered to platelets less than the bacterial nanocellulose tube.The whole blood coagulation test results showed that the composite tubes had stronger blood coagulation performance than the bacterial nanocellulose tube.(4)CCK-8 experiment results showed that compared with simple bacterial nanocellulose,the BNC/PDA-0.1 composite tubes could promote the proliferation of human umbilical vein endothelial cells;the other four composite tubes inhibited cell proliferation;a

关 键 词：生物材料 人工血管 细菌纳米纤维素 聚多巴胺 血液相容性 细胞相容性 组织工程 

分 类 号：R459.9[医药卫生—治疗学] R318.08[医药卫生—临床医学]