基于低温3D打印丝素蛋白/Ⅰ型胶原/羟基磷灰石支架的力学性能  被引量:2

Mechanical properties of silk fibroin/typeⅠcollagen/hydroxyapatite scaffolds based on low-temperature 3D printing

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作  者:孟露露 刘浩[3] 刘涵[3,4] 张军[3] 李瑞欣 高丽兰[1,2] Meng Lulu;Liu Hao;Liu Han;Zhang Jun;Li Ruixin;Gao Lilan(Tianjin Key Laboratory for Advanced Mechatronic System Design and Intelligent Control,School of Mechanical Engineering,Tianjin University of Technology,Tianjin 300384,China;National Demonstration Center for Experimental Mechanical and Electrical Engineering Education(Tianjin University of Technology),Tianjin 300384,China;The Affiliated Stomatological Hospital of Nankai University,Tianjin Stomatological Hospital,Tianjin Key Laboratory of Oral and Maxillofacial Function Reconstruction,Tianjin 300041,China;Nankai University,Tianjin 300071,China)

机构地区:[1]天津理工大学,天津市先进机电系统设计与智能控制重点实验室,天津市300384 [2]机电工程国家级实验教学示范中心(天津理工大学),天津市300384 [3]南开大学附属口腔医院,天津市口腔医院,天津市口腔功能重建重点实验室,天津市300041 [4]南开大学,天津市300071

出  处:《中国组织工程研究》2022年第22期3550-3555,共6页Chinese Journal of Tissue Engineering Research

基  金:国家自然科学基金项目(11972198),项目负责人,李瑞欣;天津市临床医学重点学科专项(HWZX001),项目负责人:刘浩;天津市重大疾病防治科技重大专项(19ZXDBSY00070),项目负责人:刘浩。

摘  要:背景:随着3D打印技术在组织工程的快速发展,通过3D打印制备出多种支架材料被广泛用于下颌骨缺损修复,3D打印技术为下颌骨缺损修复带来了新的可能。目的:采用低温3D打印技术构建三维仿生骨支架材料,精确控制支架材料内部结构,并对其进行力学性能分析。方法:在同等体积下,通过改变打印支架线束与线束的交错角度,采用低温3D打印技术分别打印不同角度(30°,45°,90°)的丝素蛋白/Ⅰ型胶原/羟基磷灰石支架与聚己内酯/羟基磷灰石支架(共6组支架)。单轴压缩力学实验以0.5%/s的压缩速率加载6组支架,压缩至30%的应变,观察应力随着应变的变化关系;应力松弛实验以0.5%/s的压缩速率分别加载至3种打印角度丝素蛋白/Ⅰ型胶原/羟基磷灰石支架10%,20%,30%的应变,松弛保持时间3h,观察应力随时间的变化关系;蠕变实验分别以2.5,3.75,5kPa的恒定压力压缩打印角度为90°的丝素蛋白/Ⅰ型胶原/羟基磷灰石支架,蠕变保持时间3 h,观察应变随时间的变化关系。结果与结论:(1)单轴压缩力学实验:对于丝素蛋白/Ⅰ型胶原/羟基磷灰石支架和聚己内酯/羟基磷灰石支架,相同压缩应变下打印角度为90°支架的杨氏模量高于打印角度为30°,45°的支架。(2)应力松弛实验:当保持压缩速率、压缩应变及压缩角度恒定时,丝素蛋白/Ⅰ型胶原/羟基磷灰石支架的应力随松弛时间的延伸先快速降低,然后缓慢降低,随着松弛时间的延长,支架在开始时间段(1600 s以内)应力下降很快,在后期时间段(3700 s)应力降低速度减小,最后趋于平缓;当保持压缩速率、压缩应变恒定时,相对于打印角度为30°,45°的支架,打印角度为90°丝素蛋白/Ⅰ型胶原/羟基磷灰石支架的应力初值及趋于平稳时的应力值较高;当压缩速率及压缩角度恒定时,随着压缩应变值增加,丝素蛋白/Ⅰ型胶原/羟基磷灰石支架的应力值增加。(3)蠕变实BACKGROUND:With the rapid development of 3 D printing technology in tissue engineering,a variety of scaffold materials prepared by 3 D printing are widely used in mandibular defect repair.3 D printing technology brings new possibilities for mandibular defect repair.OBJECTIVE:The 3 D bionic bone scaffold material was constructed by low-temperature 3 D printing technology,and the internal structure of the scaffold was precisely controlled,and the mechanical properties of the scaffold were analyzed.METHODS:Under the same volume,by changing the interlacing angle of the printed scaffold wire harness and the wire harness,low-temperature 3 D printing technology was used to print silk fibroin/typeⅠcollagen/hydroxyapatite scaffolds with different angles(30°,45°,90°)and polycaprolactone/hydroxyapatite scaffolds(a total of 6 sets of scaffolds).The uniaxial compression mechanics experiment was loaded to 6 groups of scaffolds at a compression rate of 0.5%/s,compressed to 30%strain to observe the relationship between stress and strain.The stress relaxation experiment was applied to the three printing angles of silk fibroin/collagen I/hydroxyapatite scaffolds at a compression rate of 0.5%/s at 10%,20%,and 30%strain,and the relaxation retention time was 3 hours to observe the relationship between stress and time.In the creep experiment,the silk fibroin/collagen I/hydroxyapatite scaffold with an angle of 90°was compressed and printed at a constant pressure of 2.5,3.75,and 5 k Pa,and the creep retention time was 3 hours to observe the relationship between strain and time.RESULTS AND CONCLUSION:(1)Uniaxial compression mechanics experiment:The mechanical properties of 3 D printed silk fibroin/typeⅠcollagen/hydroxyapatite scaffold and polycaprolactone/hydroxyapatite scaffold compression showed that the 90°scaffold had higher Young’s modulus than the 30°and 45°scaffolds under the same compression strain.(2)Stress relaxation experiment:When the compression rate,compression strain and compression angle were constant,the str

关 键 词:3D打印 生物支架 丝素蛋白 Ⅰ型胶原 羟基磷灰石 单轴压缩 生物力学 

分 类 号:R459.9[医药卫生—治疗学] R318.01[医药卫生—临床医学]

 

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