水凝胶组织工程脂肪的血管化策略  

Hydrogel-based adipose tissue engineering strategies for vascularization

作　　者：吴姝涵 祝旭龙 白璐 张菲菲 蔺光帅 李江 杜嘉 李建辉[2] Wu Shuhan;Zhu Xulong;Bai Lu;Zhang Feifei;Lin Guangshuai;Li Jiang;Du Jia;Li Jianhui(College of Medicine,Yan’an University,Yan’an 716000,Shaanxi Province,China;Department of Surgical Oncology,Shaanxi Provincial People’s Hospital,Xi’an 710068,Shaanxi Province,China;School of Life Science and Technology,Xi’an Jiaotong University,Xi’an 710049,Shaanxi Province,China;Xi’an Medical University,Xi’an 710068,Shaanxi Province,China)

机构地区：[1]延安大学医学院,陕西省延安市716000 [2]陕西省人民医院肿瘤外科,陕西省西安市710068 [3]西安交通大学生命科学与技术学院,陕西省西安市710049 [4]西安医学院,陕西省西安市710068

出　　处：《中国组织工程研究》2022年第22期3573-3579,共7页Chinese Journal of Tissue Engineering Research

基　　金：陕西省重点科技创新团队项目(2014KCT-24),项目负责人:李建辉;陕西省自然科学基础研究计划(2020JQ-936),项目负责人:祝旭龙。

摘　　要：背景:当前,从种子细胞、支架材料及细胞外微环境三方面着手构建工程化脂肪组织,是软组织缺损修复及重建最具发展潜力的方向之一,但由于缺乏有效的血管生成及血液供应,移植物再吸收一直是脂肪组织工程广泛应用的主要限制。目的:对基于水凝胶支架的组织工程脂肪如何建立血管网络、有效达到血管化的相关研究予以综述。方法:利用计算机检索CNKI中国知网、万方医学网、PubMed数据库2010年1月至2020年6月发表的相关文章,检索词为"组织工程,脂肪组织,血管化,水凝胶,脂肪干细胞,Adipose-derived stem cell,tissue engineering,adipose tissue,hydrogel,vascularization"。总结相关文章,最终共纳入88篇文献进行结果分析。结果与结论:利用水凝胶材料构建的组织工程化脂肪,其血管化策略主要有以下4种:使用组织工程室模型;将脂肪干细胞与血管内皮细胞共培养;水凝胶支架材料负载细胞因子促进新生血管形成;应用具有血管生成特性的水凝胶材料。因此,应用水凝胶材料建立高度仿生化的脂肪组织单元,结合有效的血管化技术手段,将进一步提高软组织的修复重建效果。BACKGROUND:At present,the construction of engineered adipose tissue through seed cells,scaffold materials and extracellular microenvironment is one of the most potential directions for use in soft-tissue defect reconstructions.However,graft resorption attributed to lack of effective vascular network and blood supply is a major limitation for wider application of adipose tissue engineering.OBJECTIVE:To review the related research on how to establish a blood vessel network and effective engineered adipose tissue vascularization based on hydrogel scaffold.METHODS:The computer was used to search for articles on CNKI,Wanfang,and PubMed databases from January 2010 to June 2020.The search terms were"tissue engineering,adipose tissue,adipose-derived stem cell,hydrogel,vascularization"in Chinese and English.Relevant articles were summarized and finally 88 articles were included to analyze the results.RESULTS AND CONCLUSION:There are four main vascularization strategies for tissue engineered fat constructed with hydrogel materials:Tissue engineering chamber model;adipose-derived stem cells co-cultured with vascular endothelial cells;hydrogel scaffolds loaded with cytokines;application of suitable hydrogel scaffold with angiogenic properties.Therefore,the application of hydrogel materials to establish highly biomimetic adipose tissue units,combined with effective vascularization techniques,will further improve the efficiency of soft tissues repair and reconstruction.

关 键 词：组织工程 脂肪 脂肪组织 支架材料 水凝胶 血管化 脂肪干细胞 综述 

分 类 号：R459.9[医药卫生—治疗学] R318.08[医药卫生—临床医学]