骨质疏松症小鼠脂肪干细胞结合双相磷酸钙陶瓷修复骨质疏松症小鼠颅骨缺损  

Repair of calvarial defects in osteoporotic mice by adipose-derived stem cells combined with biphasic calcium phosphate ceramic scaffold

作　　者：敬劲 赵珊笛 陈龙 彭双麟 唐辉 郭代金 曾馨仪 肖金刚 Jing Jin;Zhao Shandi;Chen Long;Peng Shuanglin;Tang Hui;Guo Daijin;Zeng Xinyi;Xiao Jingang(College of Stomatology,Southwest Medical University,Luzhou 646000,Sichuan Province,China;College of Clinical Medicine,Southwest Medical University,Luzhou 646000,Sichuan Province,China;Orofacial Reconstruction and Regeneration Laboratory of Southwest Medical University,Luzhou 646000,Sichuan Province,China)

机构地区：[1]西南医科大学口腔医学院,四川省泸州市646000 [2]西南医科大学临床医学院,四川省泸州市646000 [3]西南医科大学附属口腔医院口颌面修复重建和再生实验室,四川省泸州市646000

出　　处：《中国组织工程研究》2022年第1期90-95,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(81870746,81371125),项目负责人:肖金刚;四川省科技厅项目(2014JY0044),项目负责人:肖金刚;国家级大学生创新训练项目(201810632052X),项目负责人:敬劲;泸州市人民政府-西南医科大学科技战略合作项目[2015LZCYD-S05(2/12)],项目负责人:肖金刚。

摘　　要：背景:相关研究表明双相磷酸钙可有效重建骨质疏松症伴颅骨极量缺损动物模型的骨质缺损,另有研究展望了脂肪干细胞在骨质疏松性骨折及其继发骨缺损防治中的潜在应用前景,课题组前期研究结果表明,相较于正常脂肪干细胞,骨质疏松症小鼠脂肪干细胞(osteoporosis adipose-derived stem cells,OP-ASCs)的体外增殖能力及成骨分化潜能显著降低。目的:探讨OP-ASCs结合双相磷酸钙对骨质疏松症小鼠颅骨极量缺损的重建效果。方法:18只C57BL/6雌性小鼠随机均分为3组:空白组、双相磷酸钙组、OP-ASCs/双相磷酸钙组,建立骨质疏松症小鼠颅骨极量缺损模型,空白组不植入材料,另两组分别植入双相磷酸钙、OP-ASCs/双相磷酸钙复合体。各组于第8周及12周分别处死3只小鼠,采用Micro-CT、苏木精-伊红染色、Masson染色检测其颅骨缺损部位骨形成差异。结果与结论:①术后第8周及第12周,空白组仅在颅骨缺损边缘可见少量新生骨组织,而另两组颅骨缺损部位可见明显新生骨组织,且OP-ASCs/双相磷酸钙组新生骨组织明显多于双相磷酸钙组(P<0.05);②第12周,双相磷酸钙组及OP-ASCs/双相磷酸钙组新生骨组织均明显多于第8周(P<0.05),且第12周OP-ASCs/双相磷酸钙组新生骨组织与双相磷酸钙组的差距较第8周更为明显(P<0.05);(3)结果表明,OP-ASCs/双相磷酸钙复合体对骨质疏松症小鼠颅骨极量缺损具有良好的重建效果,且重建效果较单纯双相磷酸钙更佳。BACKGROUND:Researches have shown that biphasic calcium phosphate can be used as an effective bone substitute for the repair of critical-size defects in animal models with osteoporosis,and osteoporosis adipose-derived stem cells(OP-ASCs)is promising for medical applications in patients with osteoporotic fractures and its secondary bone defects.The previous research results of the research group showed that compared with normal adipose-derived stem cells,the in vitro proliferation and osteogenic differentiation potential of OP-ASCs from osteoporosis mice were obviously reduced.OBJECTIVE:To investigate the effect of OP-ASCs combined with biphasic calcium phosphate on the repair of calvarial critical-size defects in osteoporotic mice.METHODS:Eighteen C57BL/6 female mice were divided into three groups randomly:blank group,biphasic calcium phosphate group,and OP-ASCs/biphasic calcium phosphate group.The models of osteoporosis with calvarial critical-size defects were established in each group,and then the blank group was not implanted with materials.The biphasic calcium phosphate and OP-ASCs/biphasic calcium phosphate groups were implanted with biphasic calcium phosphate ceramic scaffolds only,and OP-ASCs/biphasic calcium phosphate admixtures separately.The mice were sacrificed at the 8(n=3)and 12(n=3)weeks.The differences in bone formation at the calvarial defects were detected by micro-CT,hematoxylin-eosin staining,and Masson Trichrome staining.RESULTS AND CONCLUSION:(1)There was a small amount of bone formation in the blank group both at 8 and 12 weeks after implantation,and the new bone tissue grown well in both the biphasic calcium phosphate group and the OP-ASCs/biphasic calcium phosphate group;the OP-ASCs/biphasic calcium phosphate group achieved more bone formation than that of the biphasic calcium phosphate group(P<0.05).(2)Compared with that at 8 weeks,both the biphasic calcium phosphate group and the OP-ASCs/biphasic calcium phosphate group showed more bone formation at 12 weeks(P<0.05),and the difference of

关 键 词：脂肪干细胞 骨质疏松症 卵巢切除术 组织块贴壁法 双相磷酸钙 颅骨 极量缺损 骨组织工程 

分 类 号：R446[医药卫生—诊断学] R318[医药卫生—临床医学]