长链非编码RNA对骨相关细胞增殖、分化、凋亡的调控  

Regulation of proliferation,differentiation and apoptosis of bone-related cells by long-stranded noncoding RNA

作　　者：冯志国 孙海飚[1] 韩晓强[1] Feng Zhiguo;Sun Haibiao;Han Xiaoqiang(First Hospital of Shanxi Medical University,Taiyuan 030001,Shanxi Province,China)

机构地区：[1]山西医科大学第一医院,山西省太原市030001

出　　处：《中国组织工程研究》2022年第1期112-118,共7页Chinese Journal of Tissue Engineering Research

基　　金：省重点研发计划(社会发展领域)[晋财教(2018)349号188805#],项目负责人:孙海飚;重点研发计划(社会发展领域)[晋财教(2019)150-3号193326#],项目负责人:韩晓强。

摘　　要：背景:长链非编码RNA是控制基因表达并影响多种生物学过程的重要表观遗传调控因子。越来越多的证据表明长链非编码RNA对骨髓间充质干细胞、成骨细胞、破骨细胞和软骨细胞的增殖、分化和凋亡具有潜在的调控作用。目的:就长链非编码RNA对骨髓间充质干细胞、成骨细胞、破骨细胞和软骨细胞功能的影响、长链非编码RNA的调控机制及参与的信号通路等方面的最新研究进展作一综述,以期为骨质疏松症、骨关节炎疾病的治疗提供新的理论基础。方法:以"Long non-coding RNA;Bone marrow mesenchymal stem cells;Osteoblasts;Osteoclasts;Chondrocytes;Differentiation;Signal pathway"为英文检索词,检索PubMed、Biosos Preview及Web of Science数据库从建库至2020年8月的相关文献。制定入选标准,通过阅读文献文题、摘要和全文进行筛选,最终纳入相关68篇文献。结果与结论:①长链非编码RNA作为一种具有多种生物学功能的关键调控分子,通过多种机制调控不同的信号通路从而调控髓间充质干细胞、成骨细胞、破骨细胞和软骨细胞的增殖、分化和凋亡等生理病理过程,抑制或促进骨质疏松症、骨关节炎的进程;②常见的长链非编码RNA调节的信号通路有WNT、TGF-β/BMPs、NOTCH及PI3K/AKT信号通路,信号通路之间往往存在串扰,共同参与对骨细胞代谢的调控;编码RNA和非编码RNA之间通过竞争miRNA结合位点来相互调节。BACKGROUND:Long-stranded non-coding RNA is an important epigenetic regulator that controls gene expression and affects a variety of biological processes.More and more evidence shows that long-stranded non-coding RNA plays a potential role in regulating the proliferation,differentiation and apoptosis of bone marrow mesenchymal stem cells,osteoblasts,osteoclasts and chondrocytes.OBJECTIVE:To review the latest research progress on the effects of long-stranded non-coding RNA on the function of bone marrow mesenchymal stem cells,osteoblasts,osteoclasts and chondrocytes,the regulatory mechanism of long-stranded non-coding RNA and the signal pathway involved,in order to provide a new theoretical basis for the treatment of osteoporosis and osteoarthritis.METHODS:PubMed,Biosos Preview and Web Of Science were used to search the related articles published from database inception to August 2020.The retrieval key words were"long non-coding RNA,bone marrow mesenchymal stem cells,osteoblasts,osteoclasts,chondrocytes,differentiation,signal pathway".Finally,68 articles related to this article were selected according to inclusion criteria and after screening contents,abstracts,and titles.RESULTS AND CONCLUSION:(1)Long-stranded non-coding RNA is a key regulatory molecule with multiple biological functions.Long-stranded non-coding RNA regulates different signal pathways through various mechanisms,and further regulates the proliferation,differentiation and apoptosis of medullary mesenchymal stem cells,osteoblasts,osteoclasts and chondrocytes,and inhibit or promote the process of osteoporosis and osteoarthritis.(2)Common Longstranded non-coding RNA regulatory signal pathways include WNT,TGF-β/BMPs,NOTCH and PI3K/AKT.These signaling pathways often have crosstalk,and are jointly involved in the regulation of bone cell metabolism.Coding RNA and non-coding RNA regulate each other by competing for miRNA binding sites.

关 键 词：长链非编码RNA 骨髓间充质干细胞 成骨细胞 破骨细胞 软骨细胞 分化 信号通路 

分 类 号：R446[医药卫生—诊断学] R496[医药卫生—临床医学]