ACAT1基因多态性与冠心病的关联研究  

Association between polymorphism of ACAT1 gene and susceptibility of coronary artery disease

作　　者：王永涛[1] 魏娴[1] 刘成[1] 黄定[1] 付真彦[1] 马依彤[1] WANG Yongtao;WEI Xian;LIU Cheng;HUANG Ding;FU Zhenyan;MA Yitong(Center of Heart Coronary Artery Disease,the First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,China)

机构地区：[1]新疆医科大学第一附属医院心脏中心,乌鲁木齐830054

出　　处：《新疆医科大学学报》2021年第11期1203-1208,共6页Journal of Xinjiang Medical University

基　　金：国家自然科学基金(91957208)。

摘　　要：目的探讨ACAT1基因rs1044925、rs11545566、rs12121758及rs10913733位点多态性在冠心病发病中的易感性。方法采用病例对照的研究方法,选取2017年1月-2018年12月在新疆医科大学第一附属医院心脏中心住院,经冠脉造影确诊的冠心病患者300例(冠心病组)和冠脉造影正常的健康对照者300例(对照组)为研究对象,采用改良多重连接酶反应(iMLDR)技术测定基因分型,分析ACAT1基因rs1044925、rs11545566、rs12121758及rs10913733位点多态性与冠心病的关联性。结果rs11545566位点基因型、显性模型在冠心病组和对照组间分布差异具有统计学意义(P=0.007,P=0.002);多因素分析显示在调整了混杂因素后,携带GA基因型及GG基因型者患冠心病的风险分别是AA基因型携带者的2.033倍、2.091倍(P=0.004,P=0.005),显性模型中携带GA和GG基因型者患冠心病的风险是AA基因型携带者的2.057倍(P=0.002);rs12121758位点两组间基因型、显性模型、隐性模型的分布差异具有统计学意义(P=0.016,P=0.010,P=0.041)。rs10913733位点两组间基因型、显性模型、隐性模型的分布差异具有统计学意义(P=0.021,P=0.023,P=0.022)。rs11545566位点GA+GG基因型在女性组、不吸烟组、高血压组、糖尿病组及非糖尿病组中均显著增加了发生冠心病的风险(P均<0.05)。结论ACAT1基因rs11545566位点GA及GG基因型携带者患冠心病的风险显著增加,提示ACAT1基因rs11545566位点可作为冠心病的潜在生物标记物。Objective To investigate the association between ACAT1 gene polymorphism(rs1044925,rs11545566,rs12121758,rs10913733)and the susceptibility of coronary artery diseases(CAD).Methods A case-control study was conducted to select patients who underwent coronary angiography in the First Affiliated Hospital of Xinjiang Medical University from January 2017 to December 2018,300 patients with CAD diagnosed by coronary angiography and 300 healthy controls with normal coronary angiography were selected.The genotype was determined by improved multiple ligase reaction(iMLDR)technology,and the association between rs1044925,rs11545566,rs12121758,rs10913733 lo⁃ci of ACAT1 and the risk of CAD were analyzed.Results For rs11545566,the distribution frequency of the three geno⁃types and dominant model showed a significant difference between the CAD and control subjects(P=0.007,P=0.002).Multivariate analysis showed that the risk of coronary heart disease was 2.033 times and 2.091 times for GA genotype and GG genotype respectively(P=0.004,P=0.005)after adjustment for confounding factors,in the dominant model,the risk of coronary heart disease was 2.057 times higher in GA and GG genotype than in AA genotype(P=0.002).For rs12121758 and rs10913733,the distribution frequency of the three genotypes,dominant model and recessive model showed a significant difference between the CAD and control subjects(rs12121758:P=0.016,P=0.010,P=0.041;rs10913733:P=0.021,P=0.023,P=0.022).We also found that the GA+GG genotype of rs11545566 locus significant⁃ly increased the risk of CAD in the female group,non-smoking group,hypertension group,T2DM group and non T2DM group(P<0.05).Conclusion The G allele carriers of ACAT1 gene rs11545566 locus have a significantly in⁃creased risk of CAD,suggesting that ACAT1 gene rs11545566 locus may be used as a potential biomarker of CAD.

关 键 词：ACAT1 基因多态性 冠心病 关联研究 

分 类 号：R541[医药卫生—心血管疾病]