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作 者:赖晓健[1,2] 彭帅 张哲[3] 李忠琴[1,2] LAI Xiaojian;PENG Shuai;ZHANG Zhe;LI Zhongqin(Fisheries College,Jimei University,Xiamen 361021,China;Engineering Research Center of the Modern Industry Technology for Eel,Ministry of Education,Xiamen 361021,China;Fisheries Research Institute of Fujian,Xiamen 361000,China)
机构地区:[1]集美大学水产学院,福建厦门361021 [2]鳗鲡现代产业技术教育部工程研究中心,福建厦门361021 [3]福建省水产研究所,福建厦门361000
出 处:《水产科学》2021年第6期900-904,共5页Fisheries Science
基 金:国家自然科学基金资助项目(41806193);福建省教育厅科技项目(JT180271);鳗鲡现代产业技术教育部工程研究中心开放基金资助项目(RE201902);集美大学李尚大基金资助项目(ZC2018017)。
摘 要:在水温(21±1)℃和盐度35下,将体质量(266.45±18.65)g的鳗鲡雄鱼放入直径1.5m、高1.0m、水量1000L的圆形养殖桶中,每桶10尾,水中甲基睾酮质量浓度为50μg/L,对照组加入5mL无水乙醇,不投喂,每5d换水1/2。45d通过组织切片观察性腺组织结构和测定性腺发育相关激素水平,研究甲基睾酮对雄鱼性腺发育的促进作用及可能的机制。试验结果显示,暴露45d后,雄鱼性腺指数为(1.63±0.68)%,显著高于对照组(P<0.05),约为对照组的8倍。试验组雄鱼精巢为乳白色片状,处于第Ⅳ期、精子生成期(S5);对照组精巢为红色细带状,边缘为锯齿状,处于第Ⅱ期、精原细胞增殖期(S1)。试验组血清雌二醇质量浓度为(182.06±30.52)pg/mL,显著高于对照组(P<0.05);11-酮基睾酮质量浓度为(7.30±2.15)pg/mL,与对照组无显著差异。试验结果表明,甲基睾酮可作用于鳗鲡精巢并促进精子的发生。The male Japanese eel Anguilla japonica with body weight of(266.45±18.65)g was held in a round culture bucket with a diameter of 1.5 m,a height of 1.0 m and a water volume of 1000 L at a rate of 10 fish and exposed to 17α-methyltestosterone concentration of 50μg/L and to 5 mL of absolute ethanol in the control group at water temperature of(21±1)℃and a salinity of 35 for 45 d,without feeding,and with half exchange of the water every 5 days.After 17α-methyltestosterone exposure,the histological sections of Japanese eel testis were examined and serum 11-ketotestosterone(11-KT)and estradio(E2)levels were determined by ELISA to investigate the promoting effect of 17α-methyltestosterone on gonadal development and possible mechanism.The results showed that the male Japanese eel exposed to 17α-methyltestosterone concentration,with white and flaky testis at phaseⅣ/spermiogenesis stage(S5),had gonado-somatic index of(1.63±0.68)%,significantly higher than the eel in the control(about 8 folds)did,with thin testis with serrated edge at phaseⅡ/spermatogonial proliferation stage(S1)in the controls group.There was significantly higher serum E2 level in the male Japanese eel exposed to 17α-methyltestosterone [(182.06±30.52) pg/mL] than that in the control group(P<0.05),without significant change in serum 11-KT level between the male Japanese eel exposed to 17α-methyltestosterone[(7.30±2.15)pg/mL]and male Japanese eel in the control group,indicating that MT might induced spermatogenesis in Japanese eel by exposure.
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