锦鲤与草金鱼杂交子代肠道形态和酶学特性  

Differences in Intestinal Morphology and Enzymatic Characteristics between Koi and Goldfish in Hybrid and Self-Crossed Offsprings

作　　者：刘君恒 冯晓飒 张源[1,2,3] 高建忠[1,2,3] LIU Junheng;FENG Xiaosa;ZHANG Yuan;GAO Jianzhong(National Experimental Teaching Demonstration Center of Fisheries Science,Shanghai Ocean University,Shanghai 201306,China;Shanghai Aquaculture Engineering Technology Research Center,Shanghai Ocean University,Shanghai 201306,China;Key Laboratory of Freshwater Aquatic Genetic Resources,Ministry of Agriculture and Rural Affairs,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海海洋大学,水产科学国家级实验教学示范中心,上海201306 [2]上海海洋大学,上海水产养殖工程技术研究中心,上海201306 [3]上海海洋大学,农业农村部淡水水产种质资源重点实验室,上海201306

出　　处：《水产科学》2021年第6期905-910,共6页Fisheries Science

摘　　要：通过对昭和锦鲤与长尾草金鱼正交、反交及自交子代的肠道特性进行比较分析,以期为锦鲤与草金鱼的远缘杂交生产和饲养提供理论依据。首先测定昭和锦鲤与长尾草金鱼正交、反交及自交子代的肠道参数,并进行组织形态差异分析,然后测定比较F_(1)子代肠道环境pH和消化酶活性。研究结果表明,昭和锦鲤与长尾草金鱼正、反交F_(1)代在相对肠长、相对肠密度、相对肠质量、Zihler's指数上均介于亲本自交F_(1)代之间;且前肠pH也介于昭和锦鲤与长尾草金鱼自交F_(1)代之间;但正、反交F_(1)代肠道淀粉酶、脂肪酶活性显著高于二者自交F_(1)代,而蛋白酶活性介于自交F_(1)代之间。昭和锦鲤与长尾草金鱼正、反交子代肠道参数和功能高于或介于二者自交子代之间,综合了二者的肠道性状。By comparing the intestinal characteristics of the orthogonal,reciprocal and self cross offsprings of Showa koi Cyprinus carpio and long tail grass goldfish Carassius auratus,we hope to produce and study the distant hybridization of koi and goldfish to provide theoretical basis.In this study,the intestinal parameters of the orthogonal,reciprocal,and self-crossed progeny of the Showa koi and the long tail goldfish were measured,and the morphological differences were analyzed;then the pH and digestion of the intestinal environment of the F_(1) progeny were measured and compared enzyme activity.The results showed that the reciprocal F_(1) generations were in intermediate between the parental inbred F_(1) generations in relative intestinal length,relative intestinal density,relative intestinal mass,and Zihler′s index;and the pH of the foregut was also in intermediate between Showa koi and long tail grass goldfish self-breeding between F_(1) generations;however,the intestinal amylase and lipase activities of reciprocal F_(1) generations were significantly higher than those of self-breeding F_(1) generations,while protease activity was in intermediate between self-breeding F_(1) generations.The intestinal parameters of the progeny and the reciprocal progeny of the Showa koi and long tail goldfish were higher than or between those of the two self-crossed progeny,and the intestinal traits of the two were integrated.

关 键 词：昭和锦鲤 长尾草金鱼 正交 反交 肠道 

分 类 号：S917.4[农业科学—水产科学]