海水小球藻粗多糖对日本蟳免疫相关酶活性的影响  

Influence of Polysaccharide of Green Alga Chlorella pacifica on Activities of Immune-Related Enzymes in Japanese Stone Crab Charybdis japonica

作　　者：王家新 苏宏 王卫兵 许永忠 郭晨宇 顾小朵 王洪斌 WANG Jiaxin;SU Hong;WANG Weibing;XU Yongzhong;GUO Chenyu;GU Xiaoduo;WANG Hongbin(College of Marine Life and Fisheries Sciences,Jiangsu Ocean University,Lianyungang 222005,China;Xuzhou Aquarium,Xuzhou 221006,China;Suqian Dajiang Feed Co.,Ltd.,Suqian 223900,China)

机构地区：[1]江苏海洋大学海洋生命与水产学院,江苏连云港222005 [2]江苏省徐州市水族展览馆,江苏徐州221006 [3]江苏省宿迁市大江饲料有限公司,江苏宿迁223900

出　　处：《水产科学》2021年第6期917-921,共5页Fisheries Science

基　　金：江苏省科技厅—苏北科技专项项目(LYG-SZ201816);江苏省大学生创新计划项目(2020035).

摘　　要：用热水浸提法并经乙醇沉淀得到海水小球藻多糖。试验组用无菌微量注射器从日本蟳第三步足基部注入200μL海水小球藻多糖溶液,生理盐水组日本蟳注射等量生理盐水,另设未注射组;5、24、48、72、96h时,随机取1只日本蟳,用无菌蓝芯注射器自第三步足基部采血,测定日本蟳血清中超氧化物歧化酶、过氧化氢酶和溶菌酶活性。试验结果显示,试验组注射后72h日本蟳血清中超氧化物歧化酶活性达到最高,高于生理盐水组43.3%,高于未注射组45.2%;试验组注射后48h过氧化氢酶活性达到最高,分别高于生理盐水组和未注射组79.3%和80.8%;试验组注射后48h溶菌酶活性达到最高,分别高于生理盐水组和未注射组42.1%和46.1%。试验组72h后超氧化物歧化酶、过氧化氢酶和溶菌酶的活性均明显下降,而过氧化氢酶、溶菌酶下降幅度较小,72h仍明显高于对照组,96h均恢复至对照组水平。注射生理盐水的日本蟳血清中免疫活性因子比较稳定,24h后与未注射组相比,血清中免疫活性酶活性略有升高,其他时间无变化。在人工注射定量海藻多糖72h内,日本蟳机体内的免疫活性因子发生明显变化,血清中超氧化物歧化酶、过氧化氢酶和溶菌酶的活性在不同时间段内均表现出显著提高的趋势,说明海水小球藻多糖对日本蟳具有较强诱导免疫应答作用。Polysaccharides were prepared from green alga Chlorella pacifica by hot water extraction and ethanol precipitation.Activities of some immune-related enzymes including superoxide dismutase(SOD),catalase(CAT)and lysozyme(LZM)were determined in Japanese stone crab Charybdis japonica injected with 200μL of chlorella polysaccharide solution from the third walking leg base by a sterile microsyringe at 5,24,48,72,and 96 h after polysaccharide solution administration.The Japanese stone crab in control group was injected with the same amount of normal saline or without any injection to investigate influence of polysaccharide of green alga C.pacifica on activities of immune-related enzymes in Japanese stone crab.The results showed that there was the maximum activity of SOD 72 h after chlorella polysaccharide injection,43.3%higher than that in the normal saline group and 45.2%higher than that in the uninjection group.The peak CAT activity was found in 48 h,79.3%and 80.8%higher than those in the normal saline group and the uninjected group,respectively;the maximum activity of LZM was also shown in 48 h,42.1%and 46.1%higher than that in the normal saline group and the uninjected group,respectively.In 72 h,the activities of SOD,CAT and LZM were all decreased significantly.The activities of CAT and LZM were all returned to the control level at 96 h,though the activities of CAT and LZM were still significantly higher than that in control group in 72 h.Immunoreactive factors in the control group with normal saline were relatively stable,slightly increased after 24 hours compared with uninjected control group,without change at other time.Significant changes in the immunoreactive factors were observed in the Japanese stone crab injected with microalgal polysaccharides within 72 hours(P<0.05),and the activities of SOD,CAT and LZM showed significant increase in different time periods,indicating that chlorella polysaccharides had a stronger effect on immune response to Japanese stone crab.

关 键 词：海水小球藻 微藻多糖 日本蟳 免疫活性酶 

分 类 号：S917.4[农业科学—水产科学]