山东省人感染猪链球菌基因组特征分析  

Genomic investigation of human Streptococcus suis infection in Shandong Province

作　　者：胡彬[1] 王建平[2] 徐迎春[3] 刘军[4] 李涛 贾静[6] 姜文国[7] 毕秀娟[8] 曲新艺 寇增强[1] 房明[1] 孙娜[1] 杨莹 康殿民[1] 侯配斌[1] Hu Bin;Wang Jianping;Xu Yingchun;Liu Jun;Li Tao;Jia Jing;Jiang Wenguo;Bi Xiujuan;Qu Xinyi;Kou Zengqiang;Fang Ming;Sun Na;Yang Ying;Kang Dianmin;Hou Peibin(Shandong Center for Disease Control and Prevention,Jinan 250014,China;Institute for Communicable Disease Prevention and Control,Chinese Center for Disease Control and Prevention,Beijing 102206,China;Yantai Center for Disease Control and Prevention,Yantai 264003,China;Zibo Center for Disease Control and Prevention,Zibo 255026,China;Linyi Center for Disease Control and Prevention,Linyi 276000,China;QingDao Center for Disease Control and Prevention,Qingdao 266033,China;Jining Center for Disease Control and Prevention,Jining 272000,China;Taian Center for Disease Control and Prevention,Taian 271000,China;Weihai Center for Disease Control and Prevention,Weihai 264200,China)

机构地区：[1]山东省疾病预防控制中心,济南250014 [2]中国疾病预防控制中心传染病预防控制所,北京102206 [3]烟台市疾病预防控制中心,264003 [4]淄博市疾病预防控制中心,255026 [5]临沂市疾病预防控制中心,276000 [6]青岛市疾病预防控制中心,266033 [7]济宁市疾病预防控制中心,272000 [8]泰安市疾病预防控制中心,271000 [9]威海市疾病预防控制中心,264200

出　　处：《中华预防医学杂志》2021年第10期1232-1239,共8页Chinese Journal of Preventive Medicine

基　　金：山东预防医学会智飞疾病预防控制技术研究基金项目(LYH2017-03);山东省医药卫生科技发展计划项目(2017WS455);国家科技重大专项(2018ZX10713001-002)。

摘　　要：对山东省人感染猪链球菌菌株开展基因组流行病学及病原学分析,为制定合理、精准的猪链球菌预防、控制措施提供依据。本研究对分离的猪链球菌菌株开展分子分型、基因组系统进化树、毒力基因型别、耐药谱、耐药基因及其传播元件等方面的分析,并通过比较菌株刺激宿主细胞产生细胞因子的能力评价不同菌株的致病力。结果显示,血清2型、ST1型是山东患者来源猪链球菌的优势型别,其主要临床表现为脑膜炎。系统进化树分析表明山东菌株主要由5个分枝组成。山东菌株均属高致病型菌株,但细胞因子试验表明分枝2的菌株致病力显著强于其他山东菌株。除分枝3的SD2与SD4菌株外,其余山东菌株均携带不同种类的耐药基因及其传播元件。综上,山东猪链球菌的来源及进化途径存在多样性的特征,且致病能力有显著差异,需要在基因组水平开展菌株监测的工作。To investigate Streptococcus suis(S.suis)isolated from patients in Shandong province using genomic epidemiology and pathogenologic analysis.To provide the foundation to establish reasonable and accurate prevention and control measures of human S.suis infection.Molecular typing,whole genome phylogenetic tree,virulence gene typing,antibiotic resistance profile and mobile genetic elements carrying antibiotic resistance genes of isolated S.suis strains were investigated.The pathogenicity of isolated strains was also evaluated by comparing their capacity to induce pro-inflammatory cytokine production in vitro.S.suis infections in Shandong province were predominantly due to serotype 2 and sequence type 1 strains.The major symptoms were meningitis.The studied strains could be divided into five lineages.All strains belong to highly pathogenic type in Shandong province,Strains from lineage 2 possessed higher capacity to stimulate pro-inflammatory cytokine production than other strains did,even though other strains belong to highly pathogenic strains.In addition,multiple antibiotic resistance genes and corresponding mobile genetic elements werewidespread in S.suis strains from Shandong province,except strains from lineage 3.High diversities in genome,evolutionary path and pathogenicity of S.suis strains from Shandong province were revealed.It was necessary to surveillant the S.suis strain in genomic level.

关 键 词：猪链球菌 脑膜炎 基因组系统进化树 高致病型 

分 类 号：R181.3[医药卫生—流行病学] R515[医药卫生—公共卫生与预防医学]