弹力蛋白酶灌注诱导小鼠腹主动脉瘤模型建立及机制研究  

The study of the mechanism related to abdominal aortic aneurysm formation in elastase-induced aneurysm mice model

作　　者：李光[1] 李平[1] 张帅 尤斌[1] Li Guang;Li Ping;Zhang Shuai;You Bin(Department of Cardiac Surgery,Beijing Anzhen Hospital,Capital Medical University,Beijing 100029,China)

机构地区：[1]首都医科大学附属北京安贞医院心外科,100029

出　　处：《中华胸心血管外科杂志》2021年第10期618-622,共5页Chinese Journal of Thoracic and Cardiovascular Surgery

基　　金：国家自然科学基金(81870263)。

摘　　要：目的通过弹力蛋白酶灌注法构建小鼠腹主动脉瘤模型,为腹主动脉瘤形成相关机制研究提供参考。方法选用C57BL/6野生型小鼠构建猪胰蛋白酶(PPE)灌注模型,对照组用生理盐水(Saline)进行灌注,灌注后14天解剖比较腹主动脉直径的变化,HE染色并测量腹主动脉管腔直径,弹力板染色观察腹主动脉壁弹力板的破坏情况,TUNEL法来评估动脉瘤组织中的细胞凋亡情况。结果通过猪胰蛋白酶(PPE)灌注法成功构建了小鼠腹主动脉瘤模型,术后14天成瘤率100%,建模后小鼠腹主动脉直径明显增加,高于Saline对照组(P<0.05);PPE组观察到主动脉壁弹力板拉直、变薄,并出现中断现象;PPE组TUNEL阳性细胞比例明显高于Saline对照组(P<0.05)。结论弹力蛋白酶灌注法能够较为稳定地构建腹主动脉瘤模型,我们在模型中观察到腹主动脉壁中层弹力板的破坏及细胞凋亡过程的上调。为更进一步研究腹主动脉瘤发病机制提供参考。Objective To establish a mouse model of the abdominal aortic aneurysm by elastase perfusion and to provide a reference for the study of the mechanism related to abdominal aortic aneurysm formation.Methods AAAs were induced by porcine pancreatic elastase(PPE)infusion in male C57BL/6 mice.The control group was perfused with normal saline(Saline).The changes in abdominal aortic diameter were compared at 14 days after perfusion.The diameter of the abdominal aorta stained with HE was measured.The destruction of the elastic plate in the abdominal aortic wall was observed by elastic plate staining.TUNEL assay was used to evaluate the apoptosis in aneurysm tissues.Results Porcine pancreatic elastase(PPE)perfusion successfully established the mouse model of abdominal aortic aneurysm,in which an aneurysm formation rate was 100%at 14 days after the operation.After modelling,the abdominal aorta diameter in the mouse was significantly increased,higher than that in the control group perfused with normal saline(P<0.05).In the PPE group,the elastic plate of the aortic wall was straightened and thinned,and interrupted.The proportion of TUNEL-positive cells in the PPE group was significantly higher than that in the control group perfused with normal saline(P<0.05).Conclusion Elastase perfusion can stably establish the abdominal aortic aneurysm model,and we observe the destruction of the elastic plate in the medial layer of the abdominal aortic wall and the up-regulation of the apoptosis process in the model.It provides a reference to study the pathogenesis of abdominal aortic aneurysm further.

关 键 词：腹主动脉瘤 小鼠模型 猪胰弹性蛋白酶 

分 类 号：R543.16[医药卫生—心血管疾病] R-332[医药卫生—内科学]