Dendritic cell deficiencies persist seven months after SARS-CoV-2 infection  

作　　者：Alberto Pérez-Gómez Joana Vitallé Carmen Gasca-Capote Alicia Gutierrez-Valencia María Trujillo-Rodriguez Ana Serna-Gallego Esperanza Muñoz-Muela María de los Reyes Jiménez-Leon Mohamed Rafii-El-Idrissi Benhnia Inmaculada Rivas-Jeremias Cesar Sotomayor Cristina Roca-Oporto Nuria Espinosa Carmen Infante-Domínguez Juan Carlos Crespo-Rivas Alberto Fernández-Villar Alexandre Pérez-González Luis Fernando López-Cortés Eva Poveda Ezequiel Ruiz-Mateos JoséMiguel Cisneros Sonsoles Salto-Alejandre Judith Berastegui-Cabrera Pedro Camacho-Martínez Carmen Infante-Domínguez Marta Carretero-Ledesma Juan Carlos Crespo-Rivas Eduardo Márquez JoséManuel Lomas Claudio Bueno Rosario Amaya JoséAntonio Lepe Jerónimo Pachón Elisa Cordero Javier Sánchez-Céspedes Manuela Aguilar-Guisado Almudena Aguilera Clara Aguilera Teresa Aldabo-Pallas Verónica Alfaro-Lara Cristina Amodeo Javier Ampuero María Dolores Avilés Maribel Asensio Bosco Barón-Franco Lydia Barrera-Pulido Rafael Bellido-Alba Máximo Bernabeu-Wittel Candela Caballero-Eraso Macarena Cabrera Enrique Calderón Jesús Carbajal-Guerrero Manuela Cid-Cumplido Yael Corcia-Palomo Juan Delgado Antonio Domínguez-Petit Alejandro Deniz Reginal Dusseck-Brutus Ana Escoresca-Ortega Fátima Espinosa Nuria Espinosa Michelle Espinoza Carmen Ferrándiz-Millón Marta Ferrer Teresa Ferrer Ignacio Gallego-Texeira Rosa Gámez-Mancera Emilio García Horacio García-Delgado Manuel García-Gutiérrez María Luisa Gascón-Castillo Aurora González-Estrada Demetrio González Carmen Gómez-González Rocío González-León Carmen Grande-Cabrerizo Sonia Gutiérrez Carlos Hernández-Quiles Inmaculada Concepción Herrera-Melero Marta Herrero-Romero Luis Jara Carlos Jiménez-Juan Silvia Jim� 

机构地区：[1]Clinical Unit of Infectious Diseases,Microbiology and Preventive Medicine,Institute of Biomedicine of Seville(IBiS),Virgen del Rocío University Hospital,CSIC,University of Seville,Seville,Spain [2]Department of Medical Biochemistry,Molecular Biology,and Immunology,School of Medicine,University of Seville,Seville,Spain [3]Pneumology Service,Galicia Sur Health Research Instituto(IIS Galicia Sur),SERGAS-UVigo,Vigo,Spain [4]Infectious Diseases Unit,Department of Internal Medicine,Complexo HospitalarioUniversitario de Vigo,IIS Galicia Sur,SERGAS-UVigo,Vigo,Spain [5]Group of Virology and Pathogenesis,Galicia Sur Health Research Institute(IIS Galicia Sur)-Complexo Hospitalario Universitario de Vigo,SERGAS-UVigo,Vigo,Spain [6]Unit of Infectious Diseases,Microbiology,and Preventive Medicine,Virgen del Rocío University Hospital,Seville,Spain [7]Institute of Biomedicine of Seville(IBiS),Virgen del Rocío University Hospital/CSIC/University of Seville,Seville,Spain [8]Medico-Surgical Unit of Respiratory Diseases,Virgen del Rocío University Hospital,Seville,Spain [9]Unit of Emergencies,Virgen del Rocío University Hospital,Seville,Spain [10]Intensive Care Unit,Virgen del Rocío University Hospital,Seville,Spain [11]Department of Medicine,University of Seville,Seville,Spain [12]Cardiology and Cardiovascular Surgery Unit,Virgen del Rocío University Hospital,Seville,Spain [13]Rheumatology Unit,Virgen del Rocío University Hospital,Seville,Spain [14]Internal Medicine Unit,Virgen del Rocío University Hospital,Seville,Spain [15]Digestive and Hepatobiliary Unit,Virgen del Rocío University Hospital,Seville,Spain [16]Neurology and Clinical Neurophysiology Unit,Virgen del Rocío University Hospital,Seville,Spain [17]Endocrinology and Nutrition Unit,Virgen del Rocío University Hospital,Seville,Spain [18]Urology and Nephrology Unit,Virgen del Rocío University Hospital,Seville,Spain [19]Unit of Clinical Investigation and Clinical Trials,Virgen del Rocío University Hospital,Seville,Spain

出　　处：《Cellular & Molecular Immunology》2021年第9期2128-2139,共12页中国免疫学杂志（英文版）

基　　金：This work was supported by Consejeria de Transformacion Economica,Industria,Conocimiento y Universidades Junta de Andalucia(research Project CV20-85418);Consejeria de salud Junta de Andalucia(Research Contract RH-0037-2020 to JV)the Instituto de Salud Carlos III(CP19/00159 to AGV,FI17/00186 to MRJL,FI19/00083 to MCGC,CM20/00243 to APG,and COV20/00698 to support COHVID-GS);the Red Temática de Investigación Cooperativa en SIDA(RD16/0025/0020 and RD16/0025/0026);which is included in the Acción Estratégica en Salud,Plan Nacional de Investigación Científica,Desarrollo e Innovación Tecnológica,2008 to 2011 and 2013 to 2016,Instituto de Salud Carlos III,Fondos FEDER;ERM was supported by the Spanish Research Council(CSIC).

摘　　要：Severe Acute Respiratory Syndrome Coronavirus(SARS-CoV)-2 infection induces an exacerbated inflammation driven by innate immunity components.Dendritic cells(DCs)play a key role in the defense against viral infections,for instance plasmacytoid DCs(pDCs),have the capacity to produce vast amounts of interferon-alpha(IFN-α).In COVID-19 there is a deficit in DC numbers and IFN-αproduction,which has been associated with disease severity.In this work,we described that in addition to the DC deficiency,several DC activation and homing markers were altered in acute COVID-19 patients,which were associated with multiple inflammatory markers.Remarkably,previously hospitalized and nonhospitalized patients remained with decreased numbers of CD1c+myeloid DCs and pDCs seven months after SARS-CoV-2 infection.Moreover,the expression of DC markers such as CD86 and CD4 were only restored in previously nonhospitalized patients,while no restoration of integrinβ7 and indoleamine 2,3-dyoxigenase(IDO)levels were observed.These findings contribute to a better understanding of the immunological sequelae of COVID-19.

关 键 词：SARS-CoV-2 COVID-19 Dendritic cell Long-COVID 

分 类 号：R563.1[医药卫生—呼吸系统]