玉米抗粗缩病种质资源鉴定与评价  

Identification and Evaluation of Maize Germplasm Resource against Rice Black Streaked Dwarf Virus

作　　者：邸垫平[1] 路银贵[1] 张爱红[1] 杨菲[1] 田兰芝[1] 苗洪芹[1] DI Dian-ping;LU Yin-gui;ZHANG Ai-hong;YANG Fei;TIAN Lan-zhi;MIAO Hong-qin(Plant Protection Institute,Hebei Academy of Agricultural and Forestry Sciences/Integrated Pest Management Centre of Hebei Province/Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China,Ministry of Agriculture and Rural Affairs,Baoding 071000)

机构地区：[1]河北省农林科学院植物保护研究所/河北省农业有害生物综合防治工程技术研究中心/农业部华北北部作物有害生物综合治理重点实验室,保定071000

出　　处：《植物遗传资源学报》2021年第6期1615-1623,共9页Journal of Plant Genetic Resources

基　　金：国家重点研发计划(2018YFD0200608)。

摘　　要：玉米粗缩病是我国玉米种植区主要的病毒病害,发掘优异的抗性种质资源和选育优良抗病品种是防治玉米粗缩病最为经济有效的措施。本研究在粗缩病重病区采用田间自然发病方法,对201份玉米种质资源进行了连续2年的抗性鉴定和评价,同时采用人工接种方法对其中25份种质开展了进一步的鉴定。结果表明,仅沈137高抗粗缩病;P138、丹3130、辽68、齐318、黄野四、CA339、H191、齐319、X178、SH15、金黄59、R18等12份自交系表现抗病,835、中自01、9046、多黄29、CA335、金黄96B、海9-21、金黄55、沈136等9份自交系表现中抗;方差分析表明,品种抗性不同年份间的鉴定结果差异极显著(F=69.999,P<0.01)。人工接种鉴定结果表明,P138、R18表现中抗,黄野四和金黄55病情指数为46.15、82.14,表现高感粗缩病,进一步验证齐319、丹3130、X178具有稳定抗病性。筛选出的抗病种质除金黄96B外,其余均选自美国的杂交种P78599,抗粗缩病玉米自交系的筛选为抗病品种培育提供了科学依据。Maize rough dwarf disease(MRDD)is a severe virus-conferring disease in multiple maize cultivation areas in China.Exploration of resistant germplasm resource and application in resistance breeding is the most economical and effective measure.In this paper,201 maize germplasm accessions were subjected upon natural inoculation over two years in a high incidence area for MRDD.Out of them,25 accessions were tested by artificial inoculation.Under field condition,one accession Shen 137 turned to be highly resistant;12 inbred lines(P138,Dan 3130,Liao 68,Qi 318,Huangyesi,CA339,H191,Qi 319,X178,SH15,Jinhuang 59 and R18)were resistant,nine accessions(835,Zhongzi 01,9046,Duohuang 29,CA335,Jinhuang 96B,Hai 9-21,Jinhuang 55 and Shen 136)showed moderate resistance.Notably,the phenotypic datasets gained from two years showed significant different(F=69.999,P<0.01).Moreover,by tests for disease resistance by deployment of artificial inoculation,two accessions P138 and R18 which turned to be moderately resistant,whereas Huangyesi and Jinhuang 55 were highly susceptible with disease index of 46.15 and 82.14,respectively.However it was further verified that Qi 319,Dan 3130 and X178 had stable disease resistance by artificial inoculation identification.The germplasms showing resistant,except Jinhuang 96B,were selected from the hybrid P78599.Collectively,this study identified several maize inbred lines with rough dwarf disease resistance,valuable in maize breeding for MRDD resistant varieties.

关 键 词：玉米自交系 玉米粗缩病 水稻黑条矮缩病毒 抗性鉴定 

分 类 号：S435.131[农业科学—农业昆虫与害虫防治]