基于网络药理学探讨丹参-黄芪-川芎配伍治疗冠心病心绞痛的作用机制  

Study on the Mechanism of Danshen,Huangqi,and Chuanxiong in the Treatment of Angina Pectoris Based on Network Pharmacology

作　　者：张淼 李丹丹[3] 马民[2] 金宇[1] 陈启庭[1] 殷惠军[3] ZHANG Miao;LI Dandan;MA Min;JIN Yu;CHEN Qiting;YIN Huijun(Shenzhen Second People′s Hospital,Shenzhen 518000,Guangdong,China,Jinan University,Guangzhou 510632,Guangdong,China;Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing 100029,China)

机构地区：[1]深圳市第二人民医院,广东深圳518000 [2]暨南大学,广州510632 [3]中国中医科学院西苑医院,北京100029

出　　处：《中西医结合心脑血管病杂志》2021年第21期3640-3644,共5页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

基　　金：深圳医疗卫生三名工程(No.SZSM201612049)。

摘　　要：目的应用网络药理学分析丹参-黄芪-川芎配伍治疗冠心病心绞痛的作用机制。方法通过中药系统药理学数据库与分析平台(TCMSP)检索丹参、黄芪、川芎的靶点及活性成分,利用PharmgKb、TTD、DrugBank数据库获取与冠心病心绞痛相关的靶点基因,疾病/药物中的“成分靶点网络”采用Cytoscape 3.7.0软件构建,对关键活性组分进行筛选,并基于DAVID数据库分析共同靶点的京都基因与基因组百科全书(KEGG)富集通路。结果筛选得出丹参-黄芪-川芎的活性组分共34个,直接作用靶点有13个,发现丹参-黄芪-川芎中的丹参新酮、槲皮素、丹参酮ⅡA、4-亚甲丹参新酮等重要活性成分,主要作用于ADRB1、ADRA2A、ADRA2B、ADRA2C等相关靶点蛋白的调节,通过对环磷酸鸟苷(cGMP)-蛋白激酶G(PKG)信号通路、扩张型心肌病、神经活性配体-受体相互作用等信号通路的调控,发挥治疗冠心病心绞痛的作用。结论初步揭示了丹参-黄芪-川芎改善冠心病心绞痛的作用机制,为后续基础研究以及更好地开发利用治疗冠心病心绞痛的相关中药成药提供理论依据和方向。Objective To explore the mechanism of Danshen,Huangqi,and Chuanxiong in the treatment of angina pectoris based on network pharmacology.Methods The targets and active components of Danshen,Huangqi,and Chuanxiong were searched through the Traditional Chinese Medicines Systems Pharmacology Platform(TCMSP).The target genes related to angina pectoris were obtained by PharmGKB,OMIM,TTD,and DrugBank databases.Cytoscape 3.7.0 software was used to construct the"component target network".DAVID was used to perform Kyoto Encyclopedia of Genes and Genomes(KEGG)pathways enrichment analysis.Results Thirty-four active components of Huangqi,Danshen,and Chuanxiong were screened,which could directly act on 13 targets.Miltirone,quercetin,tanshinoneⅡA,4-methyl salvianone,and other important active components in Danshen,Huangqi,and Chuanxiong mainly acted on the regulation of ADRB1,ADRA2A,ADRA2B,ADRA2C,and other related target proteins.KEGG pathway enrichment yielded 3 related pathways,involving cyclic guanosine monophosphate-protein kinase G signaling pathway,dilated cardiomyopathy,and neuroactive ligand-receptor interaction.Conclusion The mechanism of Huangqi,Danshen,and Chuanxiong in the treatment of angina pectoris of coronary heart disease are preliminarily revealed,and this will provide a reference for the further basic research and better development and utilization of traditional Chinese medicine for treating coronary heart disease angina pectoris.

关 键 词：冠心病 心绞痛 丹参 黄芪 川芎 网络药理学 作用机制 

分 类 号：R28[医药卫生—中药学]