通络益肾方对单侧输尿管结扎大鼠肾纤维化及肾上腺髓质素表达的影响  

Effects of Tongluo Yishen Recipe on Renal Fibrosis and ADM Expression in UUO Rats

作　　者：李冰[1] 韩琳 韩佳丽 高誉珊 沈一凡 秦建国[1] LI Bing;HAN Lin;HAN Jiali;GAO Yushan;SHEN Yifan;QIN Jianguo(Dongfang Hospital Affilicated to Beijing University of Chinese Medicine,Beijing 100078,China;College of Chinese Medicine,Beijing University of Chinese Medicine,Beijing 100700,China;Yinghai Hospital of Daxing District,Beijing 100076,China)

机构地区：[1]北京中医药大学东方医院,北京100078 [2]北京中医药大学中医学院,北京100700 [3]北京市大兴区瀛海医院,北京100076

出　　处：《世界中医药》2021年第20期3018-3022,共5页World Chinese Medicine

基　　金：国家自然科学基金资助项目(81173407);北京市中医管理局北京中医药“薪火传承3+3工程”建设项目郭士魁名家研究室项目(2011-SZ-A-27);首都卫生发展科研专项项目(首发2016-2-4201)。

摘　　要：目的:探讨通络益肾方对单侧输尿管结扎大鼠肾间质纤维化及肾上腺髓质素(ADM)表达的影响,并探究其可能的机制。方法:将50只雄性SD大鼠按随机数字表法分为5组:假手术组、模型组、通络益肾方组、缬沙坦组、ADM观察组,每组10只。分别采取相应的干预措施。Masson染色观察大鼠肾脏的病理改变;免疫组织化学法检测大鼠肾脏E-钙黏蛋白(E-cadherin)的表达;放射免疫法检测大鼠血清ADM水平;ELISA检测大鼠血清Ⅲ型前胶原(PCⅢ)、肾组织ADM水平,并对肾组织ADM和E-cadherin进行相关性分析。结果:与假手术组比较,模型组血清ADM、PCⅢ水平明显升高(P<0.01),肾组织E-cadherin、ADM水平降低(P<0.01)。与模型组比较,通络益肾方组和缬沙坦组血清ADM、PCⅢ水平降低(P<0.01、P<0.05),肾组织E-cadherin、ADM水平升高(P<0.01、P<0.05);ADM观察组血清PCⅢ水平降低(P<0.05),血清ADM、肾组织E-cadherin、ADM水平升高(P<0.05)。其中肾组织ADM与E-cadherin水平呈显著正相关(r=0.62,P<0.01)。结论:通络益肾方能够改善肾间质纤维化模型(UUO)大鼠肾间质纤维化,抑制纤维化指标PCⅢ的表达,上调E-cadherin的表达,其作用机制可能是通过上调肾脏局部ADM蛋白表达而实现的。Objective:To investigate the effect of Tongluo Yishen Recipe(TLYSR)on renal interstitial fibrosis and ADM expression in UUO rats,and to explore its possible mechanism.Methods:A total of 50 male SD rats were randomly divided into 5 groups:a sham operation group,a model group,a TLYSR group,a valsartan group and a ADM treatment group,with 10 rats in each group.Corresponding intervention measures were taken in each group.The pathological changes of kidney were observed by Masson staining;the expression of E-cadherin in kidney was detected by IHC;the content of serum ADM was detected by radioimmunoassay;the content of serum PCⅢand ADM in renal tissue were detected by ELISA,and the correlation between ADM and E-cadherin was analyzed.Results:Compared with sham operation group,the levels of serum ADM and PCⅢin model group were significantly increased(P<0.01),and the levels of E-cadherin and ADM in renal tissue were decreased(P<0.01).Compared with the model group,the contents of serum ADM and PCⅢin TLYSR group and valsartan group were decreased(P<0.01,P<0.05),and the contents of E-cadherin and ADM in renal tissue were increased(P<0.01,P<0.05);the contents of serum PCⅢin ADM treatment group were decreased(P<0.05),and the contents of serum ADM,E-cadherin and ADM in renal tissue were increased(P<0.05),and the levels of ADM and E-cadherin in the renal tissue were positively correlated(r=0.62,P<0.01).Conclusion:TLYSR can improve renal interstitial fibrosis in UUO rats,inhibit the expression of fibrosis index PCⅢand up regulate the expression of E-cadherin,which may be achieved by up regulating the expression of ADM protein in kidney.

关 键 词：通络益肾方 肾间质纤维化 单侧输尿管梗阻 肾上腺髓质素 血清PCⅢ E-钙黏蛋白 

分 类 号：R289.5[医药卫生—方剂学]