紫草素对大鼠鼻黏膜机械性损伤的修复作用  

Effect of shikonin on wound healing of mechanical damage nasal mucosa in SD rats

作　　者：胡盛琳 晏小蓉 黄翔悦 张雪娟[1] HU Shenglin;YAN Xiaorong;HUANG Xiangyue;ZHANG Xuejuan(Ya'an People's Hospital, Ya'an 625000, Sichuan, China;College of Veterinary Medicine,Sichuan Agricultural University, Chengdu 611130,China)

机构地区：[1]雅安市人民医院,四川雅安625000 [2]四川农业大学动物医学院,四川成都611130

出　　处：《西部医学》2021年第11期1613-1617,共5页Medical Journal of West China

基　　金：雅安市科技知识产权局项目(2018yyjskf16)。

摘　　要：目的探讨紫草素对SD大鼠鼻粘膜损伤的修复作用,为临床使用紫草素治疗鼻损伤提供参考依据。方法将60只SD大鼠制备鼻粘膜机械损伤模型,按随机数字表法分为五组,每组各12只,分别为紫草素高(紫草素溶液4 mg/kg涂抹鼻腔)、中(紫草素溶液2 mg/kg涂抹鼻腔)、低剂量组(紫草素溶液1mg/kg涂抹鼻腔)、阳性药物对照组(0.1m红霉素软膏涂抹鼻腔)及模型对照组(不作任何处理),连续用药21天。观察不同时间各组动物一般情况,鼻黏膜病理及组织病理学变化。结果3种不同剂量组的紫草素均能改善鼻黏膜损伤大鼠的临床表现,差异有统计学意义(P<0.05);经不同剂量的紫草素处理后,大鼠受损鼻黏膜上皮细胞修复、中性粒细胞减少、淋巴细胞浸润降低,差异均有统计学意义(P<0.05),但中、高剂量组效果更优于低剂量组(P<0.05)。结论3种剂量的紫草素对鼻黏膜损伤的修复均有明显改善作用,中、高剂量效果更优,发挥作用与用药剂量、时间有关;其机制可能与紫草素抑制炎症反应,促进细胞增殖有关。Objective The aim of the study was to evaluate the effect of shikonin on nasal wound healing after mechanical injury in SD rats,which to provide the reference for clinical use of the drug to treat nasal mucosa injury.Methods Nasal mucosa mechanical damage model was prepared.Different doses of Shikonin(1,2,4 mg/kg)were given to the treatment groups,at the same time,model control and erythromycin as positive control group were set.The manifestations of rats in each group,and pathological and histopathological changes of nasal mucosa were observed.Results Three different doses of shikonin can improve the clinical performance of rats with nasal mucosal injury,and the difference is statistically significant(P<0.05);after treatment with different doses of shikonin,the damaged nasal mucosal epithelial cells are repaired,Neutrophils decreased,lymphocyte infiltration decreased,the difference was statistically significant(P<0.05).Conclusion The three doses of shikonin have a significant improvement effect on the repair of nasal mucosal damage.The effect is related to the dosage and time of the medication.It can be used for clinical prevention and treatment of nasal damage.

关 键 词：紫草素 SD大鼠 鼻粘膜损伤 修复 

分 类 号：R332[医药卫生—人体生理学]