细粒棘球蚴促进骨髓间充质干细胞的增殖  

Echinococcus granulosus promotes the proliferation of bone marrow mesenchymal stem cells

作　　者：梁学奇[1] 周慧[1] 武杰 习羽[1] 何家赓[1] 秦乐[1] 陈雪玲 吴向未[1] 孙凡 牛建华[1] Liang Xueqi;Zhou Hui;Wu Jie;Xi Yu;He Jiageng;Qin Le;Chen Xueling;Wu Xiangwei;Sun Fan;Niu Jianhua(Department of General Surgery,First Affiliated Hospital of Medical College of Shihezi University,Shihezi 832000,Xinjiang Uygur Autonomous Region,China;Department of Immunology,Medical College of Shihezi University,Shihezi 832002,Xinjiang Uygur Autonomous Region,China)

机构地区：[1]石河子大学医学院第一附属医院普外科,新疆维吾尔自治区石河子市832000 [2]石河子大学医学院免疫学教研室,新疆维吾尔自治区石河子市832002

出　　处：《中国组织工程研究》2022年第19期3004-3010,共7页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(81760570),项目负责人:吴向未,国家自然科学基金项目(81760371),项目负责人:陈雪玲。

摘　　要：背景:目前,国内外有关细粒棘球蚴与间充质干细胞之间相互作用影响的报道很少,细粒棘球蚴与干细胞增殖方面的研究目前还是空白。目的:探讨细粒棘球蚴对骨髓间充质干细胞增殖的影响及机制。方法:分离、培养C57BL/6小鼠骨髓间充质干细胞,分为单独骨髓间充质干细胞组、Notch信号通路特异性抑制剂DAPT组、细粒棘球蚴原头蚴组、细粒棘球蚴原头蚴+特异性抑制剂DAPT组。培养1,2,3 d采用CCK-8法检测各组骨髓间充质干细胞增殖情况,Western blot、实时荧光定量PCR检测各组骨髓间充质干细胞中Notch信号通路相关Notch-1,Jagged-1,Hes-1蛋白及mRNA表达。结果与结论:(1)与单独骨髓间充质干细胞组相比,特异性抑制剂DAPT组细胞增殖受到抑制(P<0.05),细粒棘球蚴原头蚴组细胞增殖明显(P<0.05);与特异性抑制剂DAPT组相比,细粒棘球蚴原头蚴+特异性抑制剂DAPT组细胞增殖上调(P<0.05);(2)Notch-1,Jagged-1,Hes-1的蛋白及mRNA表达:特异性抑制剂DAPT组低于单独骨髓间充质干细胞组(P<0.05);细粒棘球蚴原头蚴组高于单独骨髓间充质干细胞组(P<0.05);细粒棘球蚴原头蚴+特异性抑制剂DAPT组高于特异性抑制剂DAPT组(P<0.05);(3)结果表明,细粒棘球蚴可通过Notch信号通路促进骨髓间充质干细胞的增殖,这一现象可能为细粒棘球蚴外囊的形成机制奠定理论基础,从而解释细粒棘球蚴感染宿主后如何获得免疫逃逸,甚至可能为细粒棘球蚴病的预防和早期诊断打开一个新的研究方向。BACKGROUND:At present,there are few reports on the interaction between Echinococcus granulosus and mesenchymal stem cells at home and abroad,and the research on Echinococcus granulosus and stem cell proliferation is still blank.OBJECTIVE:To explore the effect and mechanism of Echinococcus granulosus on the proliferation of bone marrow mesenchymal stem cells.METHODS:C57 BL/6 mouse bone marrow mesenchymal stem cells were isolated and cultured,and divided into bone marrow mesenchymal stem cell group,Notch signaling pathway specific inhibitor DAPT group,Echinococcus granulosus protocercaria group,and Echinococcus granulosus protocercaria+specific inhibitor DAPT group.At 1,2,and 3 days after culture,CCK-8 assay was used to detect the proliferation of bone marrow mesenchymal stem cells in each group.Western blot assay and real-time fluorescent quantitative PCR were used to detect expression levels of Notch-1,Jagged-1,Hes-1 proteins and mRNA related to the Notch signaling pathway in bone marrow mesenchymal stem cells of each group.RESULTS AND CONCLUSION:(1)Compared with bone marrow mesenchymal stem cell group,and the proliferation was inhibited in the specific inhibitor DAPT group(P<0.05),and the proliferation of the Echinococcus granulosus protocercaria group was significant(P<0.05).Compared with the specific inhibitor DAPT group,the proliferation significantly increased in the Echinococcus granulosus protocercaria+specific inhibitor DAPT group(P<0.05).(2)The protein and m RNA expression levels of Notch-1,Jagged-1,and Hes-1 were lower in the specific inhibitor DAPT group than those in the bone marrow mesenchymal stem cell group(P<0.05).These expression levels in the Echinococcus granulosus protocercaria group were higher than those in the bone marrow mesenchymal stem cell group(P<0.05);these expression levels were higher in the Echinococcus granulosus protocercaria+specific inhibitor DAPT group than those of the specific inhibitor DAPT group(P<0.05).(3)In conclusion,Echinococcus granulosus can promote the proliferation

关 键 词：干细胞 骨髓间充质干细胞 细粒棘球蚴 增殖 NOTCH 信号通路 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学]