外泌体在激素性股骨头坏死修复信号交流网络中的调控机制  

Regulatory mechanism of exosomes in signal communication network of steroid induced avascular necrosis of the femoral head repair

作　　者：王伟伟 欧志学[2] 章晓云[1] 李时斌 周毅 李统 Wang Weiwei;Ou Zhixue;Zhang Xiaoyun;Li Shibin;Zhou Yi;Li Tong(Ruikang Hospital Affiliated to Guangxi University of Traditional Chinese Medicine,Nanning 530000,Guangxi Zhuang Autonomous Region,China;Guilin Hospital of Traditional Chinese Medicine,Guilin 541002,Guangxi Zhuang Autonomous Region,China)

机构地区：[1]广西中医药大学附属瑞康医院,广西壮族自治区南宁市530000 [2]桂林市中医医院,广西壮族自治区桂林市541002

出　　处：《中国组织工程研究》2022年第19期3056-3064,共9页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(81960803),项目参与人:章晓云;广西自然科学基金青年基金(2020GXNSFBA159053),项目负责人:章晓云;广西高校青年教师基础能力提升项目(2019KY0352),项目负责人:章晓云;广西中医药大学2019年校级科研课题(2019QN027),项目负责人:章晓云;广西中医药大学一流学科课题(2019XK026,2019XK029),项目负责人:章晓云。

摘　　要：背景:激素性股骨头坏死的患病率正逐年升高,而外泌体作为目前研究的一大热点,其在成骨、血管生成以及骨组织工程应用方面已凸显出一定的优势与价值。目的:阐述目前外泌体在激素性股骨头坏死方面的研究进展。方法:文献检索数据库包括中国知网、万方、维普、中国生物医学文献数据库、PubMed、Embase、Medline及Web of Science数据库,中文检索词为"外泌体、激素性股骨头坏死、间充质干细胞、血管、脂代谢、组织工程、成骨细胞、破骨细胞、滑膜细胞、软骨细胞、骨细胞、富血小板血浆",英文检索词为"Exosomes,Steroid induced Avascular Necrosis of the Femoral Head,Mesenchymal stem cells,Blood vessel,fat metabolism,Tissue Engineering,Osteoblast,Osteoclasts,Synovial cells,chondrocytes,Bone cells,platelet-rich plasma",时间设置为2000-2021年,根据纳入和排除标准共筛选63篇文献。结果与结论:(1)外泌体作为运输载体,将间充质干细胞、内皮细胞、成骨细胞、破骨细胞、滑膜细胞、软骨细胞以及骨细胞之间所形成的信号交流网络紧密地联系在一起,调节成骨和血管生成,从而在激素性股骨头坏死骨组织修复当中发挥着重要作用;(2)其主要通过Wnt/β-catenin和PI3K/AKT等信号通路,调控信号交流网络中的细胞增殖、凋亡及分化,从而改善激素所导致的交流网络失衡;(3)文章初步阐释了外泌体在激素性股骨头坏死中的作用情况,但由于目前相关性研究多集中于单种细胞的代谢异常,故未能系统阐明偶联机制方面的具体情况;(4)另外由于目前技术限制,外泌体对生物的绝对安全性未得到较为科学的验证,其提取、鉴定和保存对于技术、设备要求颇高,可稳定携带并持续释放外泌体的亲生物材料研发机制又尚未成熟,这些都在一定程度上限制了外泌体治疗在临床上的推广;(5)未来应基于现有的相关机制及生物信息结果,开展外�BACKGROUND:The prevalence of steroid induced avascular necrosis of the femoral head is increasing year by year,and exosomes,as a major focus of current research,have highlighted the advantages and value of their applications in osteogenesis,angiogenesis,and bone tissue engineering.OBJECTIVE:To summarize the current research progress of exosomes in steroid induced avascular necrosis of the femoral head.METHODS:CNKI,Wanfang,VIP,Chinese Biomedical Literature Database,PubMed,Embase,Medline,and Web of Science were retrieved for relevant articles.Search terms were"exosomes,steroid induced avascular necrosis of the femoral head,mesenchymal stem cells,blood vessel,fat metabolism,tissue engineering,osteoblast,osteoclasts,synovial cells,chondrocytes,bone cells,platelet-rich plasma"in Chinese and English.The time was set from 2000 to 2021.Totally,63 articles were screened according to the inclusion and exclusion criteria.RESULTS AND CONCLUSION:(1)Exosomes are used as transport vehicles to closely link the signal communication network formed between mesenchymal stem cells,endothelial cells,osteoblasts,osteoclasts,synovial cells,chondrocytes and osteocytes,and regulate osteogenesis and angiogenesis,which plays an important role in the repair of hormonal osteonecrosis of the femoral head.(2)It mainly regulates the proliferation,apoptosis,and differentiation of other cells in the signal communication network through Wnt/β-catenin and PI3 K/AKT signal pathways,thereby improving the imbalance of the communication network caused by hormones.(3)This article preliminarily explained the role of exosomes in steroid induced avascular necrosis of the femoral head.However,because the current correlation studies mostly focus on the metabolic abnormalities of a single cell,the specific situation of the coupling mechanism has not been systematically clarified.(4)In addition,due to current technical limitations,the absolute safety of exosomes to organisms has not been scientifically verified.The extraction,identification,and storage of exos

关 键 词：外泌体 激素性股骨头坏死 血管生成 骨组织工程 成骨细胞 破骨细胞 间充质干细胞 内皮细胞 

分 类 号：R459.9[医药卫生—治疗学] R363[医药卫生—临床医学]