高原鼢鼠发情周期阴道细胞变化特征及血清促性腺激素含量动态  

Characteristics of vaginal cells and dynamics of serum gonadotropin content in estrus cycle of plateau zokor(Eospalax baileyi)

作　　者：王艳莉 姚宝辉 张彩军 谭宇尘 康宇坤 张德罡[1,2,4] 苏军虎 WANG Yanli;YAO Baohui;ZHANG Caijun;TAN Yuchen;KANG Yukun;ZHANG Degang;SU Junhu(College of Grassland Science,Key Laboratory of Grassland Ecosystem(Ministry of Education),Pratacultural Engineering Laboratory of Gansu Province,Sino-U.S.Centers for Grazing land Ecosystem Sustainability,Gansu Agricultural University,Lanzhou 730070,China;Gansu Agricultural University-Massey University Research Centre for Grassland Biodiversity,Gansu Agricultural University,Lanzhou 730070,China;Engineering and Technology Research Center for Alpine Rodent Pest Control,National Forestry and Grassland Administration,Lanzhou 730070,China;Gansu Qilianshan Grassland Ecosystem Observation and Research Station,Tianzhu 733200,China)

机构地区：[1]甘肃农业大学草业学院,草业生态系统教育部重点实验室,中美草地畜牧业可持续发展研究中心,兰州730070 [2]甘肃农业大学—新西兰梅西大学草地生物多样性研究中心,兰州730070 [3]国家林业和草原局高寒草地鼠害防控工程技术研究中心,兰州730070 [4]甘肃省祁连山草原生态系统野外科学观测研究站,天祝733200

出　　处：《兽类学报》2021年第6期731-740,共10页Acta Theriologica Sinica

基　　金：国家自然科学基金(31760706);甘肃省陇原青年创新创业人才项目(LYRC2018-02);甘肃省科技计划项目(20JR10RA564)。

摘　　要：野生动物繁殖调控是动物生态学研究的核心问题之一,其目的是通过研究调控动物个体及种群繁殖因素,揭示物种繁殖调控和生存规律(孙儒泳等,1997;Zenuto et al.,2001)。一般而言,雌性生殖细胞生殖能力的获得需经历卵泡发育和卵子发生两个过程,而这两个相互依存的过程皆在发情周期完成。发情周期的启动为雌雄配子相互结合及下一代个体发育奠定基础(张嘉保和田见晖,2011)。In order to determine the change characteristics of vaginal cells in the estrus cycle and the duration of the estrus cycle,vaginal smears and HE staining methods were used to observe and count the types,morphological changes,and proportions of vaginal cells in plateau zokor(Eospalax baileyi).The hormone secretion dynamics of follicle-stimulating hormone(FSH)and luteinizing hormone(LH)in the serum of plateau zokor in the estrus cycle were determined by ELISA.The results showed that the main vaginal cells of plateau zokor were leukocytes,nuclear epithelial cells,incompletely cornified epithelial cells,and complete cornified epithelial cells.The proportion of nuclear epithelial cells in the proestrus was significantly higher than that during the other three periods(48.4±3.09)%(n=12,P<0.05).The proportion of complete cornified epithelial cells in the oestrus was the highest and significantly higher than that in the other three periods(59.73±7.59)%(n=15,P<0.05).The proportion of leukocytes in the metestrus and dioestrum was significantly higher than that in the proestrus and oestrus(P<0.05).The expression level of LH was the highest in the metestrus(4.7095±1.0940)ng/mL(n=6,P<0.05),which showed an increasing trend in proestrus and oestrus,and a decreasing trend in the metestrus and dioestrum.There was no significant difference in the expression level of FSH among the four periods(P>0.05);the estrus cycle duration of plateau zokor was 16 to 19 d,the characteristics of vaginal cell types in different stages of the estrus cycle were obvious,and the relative number was statistically significant.The study provided a basis for vaginal smear identification in the estrous cycle and reproductive physiology study of plateau zokor.

关 键 词：高原鼢鼠 发情周期 阴道细胞涂片 卵泡刺激素 黄体生成素 

分 类 号：Q494[生物学—生理学]