华蟾素通过IGF-1R信号通路对肝癌细胞增殖、迁移及侵袭的影响  

Effect of Cinobufavirin on Proliferation,Migration and Invasion of Hepatoma Cells Through IGF-1R Signaling Pathway

作　　者：杨建波 刘鹏[1] 张文兴[1] 陈青山 邓芳芳[1] YANG Jianbo;LIU Peng;ZHANG Wenxing;CHEN Qingshan;DENG Fangfang(The First Affiliated Hospital of Hunan University of Traditional Chinese Medicine,Changsha Hunan China 410007)

机构地区：[1]湖南中医药大学第一附属医院,湖南长沙410007

出　　处：《中医学报》2021年第12期2636-2641,共6页Acta Chinese Medicine

基　　金：湖南省卫生计生委科研项目(20201349)。

摘　　要：目的:探讨华蟾素通过IGF-1R信号通路对肝癌细胞增殖、迁移及侵袭的影响。方法:将肝癌HepG2细胞株随机分为不同浓度华蟾素注射液组(0.420 mg·L^(-1)、0.210 mg·L^(-1)、0.105 mg·L^(-1)、0.053 mg·L^(-1))及空白对照组,每组重复3次。待细胞融合度达80%左右时,各组培养基中加入相应浓度的药物,空白对照组加入等体积细胞培养基,培养48 h。CCK-8法检测肝癌细胞的增殖抑制率;细胞侵袭迁移实验(Transwell)检测肝癌细胞迁移及侵袭情况;Western blot法检测细胞IGF-1R、Akt、ERK蛋白表达。结果:与空白对照组比较,0.420 mg·L^(-1)华蟾素注射液组、0.210 mg·L^(-1)华蟾素注射液组、0.105 mg·L^(-1)华蟾素注射液组、0.053 mg·L^(-1)华蟾素注射液组细胞IGF-1R、Akt、ERK蛋白表达显著下调(P<0.05),12 h、24 h、48 h细胞增殖抑制率显著升高(P<0.05),细胞迁移能力和细胞侵袭能力显著降低(P<0.05);与0.053 mg·L^(-1)华蟾素注射液组比较,0.420 mg·L^(-1)华蟾素注射液组、0.210 mg·L^(-1)华蟾素注射液组、0.105 mg·L^(-1)华蟾素注射液组细胞IGF-1R、Akt、ERK蛋白表达显著下调(P<0.05),12 h、24 h、48 h细胞增殖抑制率显著升高(P<0.05),细胞迁移能力和细胞侵袭能力显著降低(P<0.05);与0.105 mg·L^(-1)华蟾素注射液组比较,0.420 mg·L^(-1)华蟾素注射液组、0.210 mg·L^(-1)华蟾素注射液组细胞IGF-1R、Akt、ERK蛋白表达显著下调(P<0.05),12 h、24 h、48 h细胞增殖抑制率显著升高(P<0.05),细胞迁移能力和细胞侵袭能力显著降低(P<0.05);与0.210 mg·L^(-1)华蟾素注射液组比较,0.420 mg·L^(-1)华蟾素注射液组细胞IGF-1R、Akt、ERK蛋白表达显著下调(P<0.05),12 h、24 h、48 h细胞增殖抑制率显著升高(P<0.05),细胞迁移能力和细胞侵袭能力显著降低(P<0.05)。结论:华蟾素能够抑制肝癌细胞的增殖、迁移及侵袭,其作用可能与抑制IGF-1R信号通路有关。Objective:To investigate the influence of Cinobufacin on the proliferation,migration,and invasion of hepatoma cells through the IGF-1 R signaling pathway.Methods:The liver cancer HepG-2 cell line was randomly divided into different concentrations of Cinobufosin injection groups(0.420 mg·L^(-1),0.210 mg·L^(-1),0.105 mg·L^(-1),0.053 mg·L^(-1)) and blank control Group,repeat 3 times for each group.When the degree of cell fusion reached about 80%,the corresponding concentration of drugs was added to the culture medium of each group,and an equal volume of cell culture medium was added to the blank control group,and cultured for 48 hours.Cell counting Kit-8(CCK-8) was used to determine the proliferation of HCC cells,Transwell assay was used to detect the migration and invasion of HCC cells,and Western blot(WB) was used to detect the expression of related proteins.Results:Compared with the blank control group,the expression of IGF-1 R,AKt,and ERK was significantly down-regulated(P<0.05) in 0.420 mg·L^(-1),0.210 mg·L^(-1),0.105 mg·L^(-1),and 0.053 mg·L^(-)1 cinocabine injection groups.The cell proliferation inhibition rate was significantly increased at 12 h,24 h,and 48 h(P<0.05),and the cell migration and cell invasion capacity were significantly reduced(P<0.05).Compared with the 0.053 mg·L^(-1) cinocobalamin injection group,the expression of IGF-1 R,AKt,and ERK protein were significantly down-regulated(P<0.05) in 0.420 mg·L^(-1),0.210 mg·L^(-1),and 0.105 mg·L^(-1) cinocobalamin injection group.The cell proliferation inhibition rate was significantly increased at 12 h,24 h,and 48 h(P<0.05),and the cell migration and cell invasion capacity were significantly reduced(P<0.05).Compared with the 0.105 mg·L^(-1) cinocobalamin injection group,the expression of IGF-1 R,AKt,and ERK protein in the 0.420 mg·L^(-1) cinocobalamin injection group and 0.210 mg·L^(-1) cinocobalamin injection group Significantly down-regulated(P<0.05),12 h,24 h,48 h cell proliferation inhibition rate was significantly increased(P<0.05),ce

关 键 词：华蟾素 IGF-1R信号通路 肝癌细胞 细胞增殖 细胞迁移 细胞侵袭 

分 类 号：R285.5[医药卫生—中药学]