益气活血通络方对骨搬移术后骨代谢及sICAM-1、sVCAM-1的影响  

Effect of Yiqi Huoxue Tongluo Recipe on Bone Metabolism,sICAM-1 and sVCAM-1 after Bone Transport

作　　者：陈江非 王新卫 张磊 倘艳锋 CHEN Jiangfei;WANG Xinwei;ZHANG Lei;TANG Yanfeng(Luoyang Orthopedic-Traumatological Hospital of Henan Province/Henan Provincial Orthopedic Hospital,Luoyang Henan China 471000)

机构地区：[1]河南省洛阳正骨医院/河南省骨科医院,河南洛阳471000

出　　处：《中医学报》2021年第12期2667-2672,共6页Acta Chinese Medicine

基　　金：河南省中医药科学研究专项课题项目(20-21ZY1061)。

摘　　要：目的:观察益气活血通络方对骨搬移术后骨代谢及人可溶性细胞间黏附分子1 (human soluble intercellular adhesion molecule 1,sICAM-1)、可溶性血管细胞黏附分子1(soluble vascular cell adhesion molecule 1,sVCAM-1)的影响。方法:50例需要骨搬移术的四肢骨髓炎或外伤性四肢骨骼缺损患者,按随机数字表法随机分为对照组和观察组,每组各25例。对照组患者给予骨搬移术;观察组在对照组治疗基础上,于骨搬移过程开始后第1天给予益气活血通络方。采用双能X线吸收测定法对患者骨搬移术后和骨折愈合过程中骨延长段的矿化骨质密度(bone mineral density,BMD)。于骨搬移过程开始第1天、第1周、第1个月、骨搬移术结束及骨质矿化结束时,采用疼痛视觉模拟评分(visual analogue scale,VAS)对患者的疼痛程度进行评估。记录患者骨折临床愈合和骨质矿化所需要的时间。分别于骨搬移术后第1周、6周和12周时,采用双抗体夹心法测定患者骨代谢相关参数的水平,包括血小板衍生生长因子(platelet derived growth factor,PDGF)、人胰岛素生长因子1(insulin growth factor 1,IGF-1)和碱性磷酸酶(alkaline phosphatase,ALP)水平,采用酶联免疫吸附法测定患者血清中sICAM-1和sVCAM-1水平。结果:观察组患者骨搬移术后第1个月、2个月、4个月,骨搬移术结束和骨质矿化结束时的延长骨段BMD水平显著高于对照组(P<0.05),且对照组和观察组患者搬移第2个月、4个月,骨搬移术结束和骨质矿化结束时的延长骨段BMD水平均显著高于骨搬移术后第1个月(P<0.05)。观察组患者骨搬移术后第1周、骨搬移术后第1个月、骨搬移结束和骨质矿化结束时的VAS显著低于对照组(P<0.05),且对照组和观察组患者搬移第1个月、骨搬移结束和骨质矿化结束时的VAS显著低于骨搬移术后第1天(P<0.05)。观察组患者骨折临床愈合和骨质矿化所需要的时间均显著低于对照组(P<0.05)。骨�Objective:To observe the effects of Yiqi Huoxue Tongluo Recipe on bone metabolism and human soluble intercellular adhesion molecule 1(sICAM-1) and soluble vascular cell adhesion molecule 1(sVCAM-1) after bone transport surgery.Methods:50 patients with extremity osteomyelitis or traumatic extremity bone defects requiring bone transport surgery were randomly divided into control group and observation group according to the random number method,with 25 cases in each group.Patients in the control group were given bone transport surgery.On the basis of the treatment of the control group,the observation group was given Yiqi Huoxue Tongluo Recipe on the first day after the surgery.The dual-energy X-ray absorptiometry was used to determine the bone mineral density(BMD) of the extended segment of the bone during the process of bone transport and fracture healing.On the first day,the first week,the first month,the end of bone transport,and the end of bone mineralization,the visual analogue scale(VAS) of pain was used to evaluate the patient′s pain degree.The time required for the clinical healing of the patient′s fracture and bone mineralization was recorded.And at 1,6,and 12 weeks after bone transfer,the double-antibody sandwich method was used to determine the levels of parameters related to bone metabolism in patients,including platelet derived growth factor(PDGF) and Insulin growth factor 1(IGF-1),and alkaline phosphatase(ALP) levels.Enzyme-linked immunosorbent assay was used to determine the levels of sICAM-1 and sVCAM-1 in patients′ serum.Results:The BMD level of the extended bone segment of the observation group was significantly higher than that of the control group at 1 month,2 months,4 months,the end of bone transport,and the end of bone mineralization(P<0.05),And the BMD level of the extended bone segment of the patients in the control group and the observation group after 2 months,4 months,the end of bone migration and the end of bone mineralization was significantly higher than that at 1 month of bone mi

关 键 词：益气活血通络方 骨搬移术 骨代谢 人可溶性细胞间黏附分子1 可溶性血管细胞黏附分子1 矿化骨质密度 

分 类 号：R274.934[医药卫生—中西医结合]