以多孔Au电极为传感界面的酶标记免疫传感器双底物研究  

A Study on the Double Substrate of Enzyme-Labeled Immunosensor with Porous Au Electrode as Sensing Interface

作　　者：何涛 毕烩元 詹颜 刘云龙 沈燕婷 沈红霞[1] He Tao;Bi Huiyuan;Zhan Yan;Liu Yunlong;Shen Yanting;Shen Hongxia(College of Biological,Chemical Science and Engineering,Jiaxing University;The Second Hospital of Jiaxing,Jiaxing,Zhejiang 314001)

机构地区：[1]嘉兴学院生物与化学工程学院 [2]嘉兴市第二医院,浙江嘉兴314001

出　　处：《嘉兴学院学报》2021年第6期43-46,73,共5页Journal of Jiaxing University

基　　金：浙江省新苗人才计划项目(2020R417034);嘉兴学院2020年校级SRT项目(CD8517203290)。

摘　　要：利用比表面较大的多孔Au电极为传感界面,构建夹心结构的电化学免疫传感器.采用双底物法对甲胎蛋白(AFP)进行检测,得到甲胎蛋白浓度在1~100 ng/mL范围内,邻苯二胺(OPD)和对氨基苯酚(PAP)的差分脉冲伏安法(DPV)响应电流线性回归方程及相关系数,检出限为0.1 ng/mL.结果发现,在双底物体系下,DPV响应电流与AFP浓度之间的线性范围和检测限基本一致,且用PAP为底物时检测的结果更明显,线性范围更宽一些.An electrochemical immunosensor with a sandwich structure is constructed by using porous Au electrode with a relatively large specific surface as sensing interface.Alpha-fetoprotein(AFP)is detected by double-substrate method.The linear regression equation and correlation coefficient of the differential pulse voltammetry(DPV)response current of o-phenylenediamine(OPD)and p-aminophenol(PAP)are obtained in the AFP concentration range of 1~100 ng/mL.The detection limit is 0.1 ng/mL.The results show that the linear range and detection limit between DPV response current and AFP concentration are basically the same in the two-substrate system,and the detection result is more obvious when PAP is used as substrate,and the linear range is wider.

关 键 词：多孔Au电极 双底物 甲胎蛋白 电化学 免疫传感器 

分 类 号：TB383[一般工业技术—材料科学与工程]