Long Chain Fatty Acid Affects Excited State Branching in Bilirubin-Human Serum Protein Complex  

长链脂肪酸影响胆红素-人血清蛋白复合物的激发态分化

作　　者：Yang-yi Liu Hai-feng Pan Jian-hua Xu Jin-quan Chen 刘阳依;潘海峰;徐建华;陈缙泉(华东师范大学,精密光谱科学与技术国家重点实验室,上海200241;山西大学极端光学协同创新中心,太原030006)

机构地区：[1]State Key Laboratory of Precision Spectroscopy,East China Normal University,Shanghai 200241,China [2]Collaborative Innovation Center of Extreme Optics,Shanxi University,Taiyuan 030006,China

出　　处：《Chinese Journal of Chemical Physics》2021年第5期621-627,I0003,共8页化学物理学报（英文）

基　　金：supported by the National Natural Science Foundation of China (No.91850202, No.21873030 and No.11674101);Shanghai Rising-Star Program(No.19QA1402800)

摘　　要：After binding to human serum albumin,bilirubin could undergo photoisomerization and photoinduced cyclization process.The latter process would result the formation of a product,named as lumirubin.These photo induced behaviors are the fundamental ofclinical therapy for neonatal jaundice.Previous studies have reported that the addition of long chain fatty acids is beneficial to the generation of lumirubin,yet no kinetic study has revealed the mechanism behind.In this study,how palmitic acid affects the photochemical reaction process of bilirubin in Human serum albumin(HSA)is studied by using femtosecond transient absorption and fluorescence up-conversion techniques.With the addition of palmitic acid,the excited population of bilirubin prefers to return to its hot ground state(S_(0))through a 4 ps decay channel rather than the intrinsic ultrafast decay pathways(<1 ps).This effect prompts the Z-Z to E-Z isomerization at the S_(0) state and then further increases the production yield of lumirubin.This is the first time to characterize the promoting effect of long chain fatty acid in the process of phototherapy with femtosecond time resolution spectroscopy and the results can provide useful information to benefit the relevant clinical study.

关 键 词：BILIRUBIN Human serum albumin Femtosecond transient absorption Excited state dynamics 

分 类 号：R341[医药卫生—基础医学]