硫化氢对过氧化氢损伤的内皮细胞eNOS和NO表达的影响  

Effect of hydrogen sulfide on expressions of eNOS and NO in endothelial cells injured by hydrogen peroxide

作　　者：张海波[1] 焦洁 李平平 宋志明 ZHANG Hai-Bo;JIAO Jie;LI Ping-Ping;SONG Zhi-Ming(The First Affiliated Hospital of Henan University,Kaifeng 475001,China)

机构地区：[1]河南大学第一附属医院,开封475001

出　　处：《中国免疫学杂志》2021年第19期2318-2321,共4页Chinese Journal of Immunology

基　　金：河南省医学科技攻关计划省部共建项目(SB201901065);河南省科技发展计划项目(182102310559,202102310373);开封市科技攻关计划项目(1903008)资助。

摘　　要：目的:探讨外源性硫化氢(H_(2)S)对过氧化氢(H_(2)O_(2))损伤的人脐静脉内皮细胞(HUVECs)的作用及一氧化氮(NO)系统的影响。方法:建立H_(2)O_(2)损伤HUVECs模型,观察H_(2)S的保护作用及对NO系统的影响。结果:HUVECs经H_(2)O_(2)处理后,贴壁细胞数目明显减少,细胞活力减低,内皮型一氧化氮合酶(eNOS)蛋白表达明显减弱,NO浓度明显减低;硫氢化钠(NaSH,H_(2)S供体)处理组贴壁细胞数目及细胞活力较H_(2)O_(2)模型组增加,eNOS蛋白表达较H_(2)O_(2)模型组明显增强,NO浓度较H_(2)O_(2)模型组明显提高。结论:H_(2)S通过调节NO系统部分改善H_(2)O_(2)诱导的HUVECs损伤。Objective:To explore the effect of exogenous hydrogen sulphide(H_(2)S)on treating human umbilical vein endothelial cells(HUVECs)injury induced by hydrogen peroxide(H_(2)O_(2))and the nitric oxide(NO)system in this procedure.Methods:Model of HUVECs injured by H_(2)O_(2)was established.The protective effect of H2S on the injured HUVECs was observed,and the expression of en-dothelial nitric oxide synthase(eNOS)and NO was detected.Results:The H_(2)O_(2)-treated HUVECs showed decrease adherent cell num-ber and NO concentration in cultured supernatant,reduced cell viability and eNOS protein.Compared with H_(2)O_(2)group,more in-creased number of adherent cells,elevated NO concentration in culture supernatant,increased cell viability and eNOS protein were observed in sodium hydrosulfide(NaHS,H_(2)S donor)group.Conclusion:Exogenous H_(2)S prevents HUVECs against H_(2)O_(2)-induced in-jury partially via the modulation of NO system.

关 键 词：硫化氢 过氧化氢 内皮细胞 一氧化氮 

分 类 号：R339.38[医药卫生—人体生理学]