SIRT6对前列腺癌细胞株增殖能力和凋亡水平的影响  

The effect of SIRT6 on the proliferative ability and apoptosis level of prostate cancer cell lines

作　　者：裴亮[1] 王振兴 周儒奎 杨李旺 储开博 武赟 翟晓艳 Pei Liang;Wang Zhenxing;Zhou Rukui;Yang Liwang;Chu Kaibo;Wu Yun;Zhai Xiaoyan(Department of Urology,The Second Hospital of Shanxi Medical University,Taiyan Shanxi 030001;Department of Basic Medicine,Shanxi University of Chinese Medicine,Jinzhong Shanxi 030619)

机构地区：[1]山西医科大学第二医院泌尿外科,山西太原030001 [2]山西中医药大学基础医学系,山西晋中030619

出　　处：《山西中医药大学学报》2021年第5期341-343,366,共4页Journal of Shanxi University of Chinese Medicine

基　　金：山西省自然基金项目(201901D211533,201901D111340);山西省中医药管理局项目(2016ZYYC13);山西中医药大学课题基金项目(2018PY-002,2019PY-133,2020PY-JC(Y)-01)。

摘　　要：目的:探讨SIRT6对前列腺癌细胞增殖能力和凋亡水平的影响。方法:采用Western印迹法观察比较SIRT6在3种前列腺癌细胞(LNCAP、PC-3、DU145)和前列腺上皮细胞(RWPE-1)中的表达水平;将PC-3细胞分为两组:SIRT6转染组(SIRT6-过表达质粒转染)和对照组(空质粒转染),用CCK-8法、Annexin V和PI双染法分别观察过表达SIRT6后对前列腺癌细胞增殖能力和凋亡水平的影响。结果:与前列腺上皮细胞比较,3种前列腺癌细胞中SIRT6的表达显著降低(P<0.01);SIRT6转染后,PC-3细胞中SIRT6表达增高(P<0.01),PC-3细胞增殖能力下降(P<0.05),凋亡水平升高(P<0.01)。结论:SIRT6可能对前列腺癌细胞的增殖能力有抑制作用,对前列腺癌细胞的凋亡有促进作用。Objective:To investigate the effect of SIRT6 on proliferation and apoptosis of prostate cancer cells.Methods:The expression levels of SIRT6 in three kinds of prostate cancer cells(LNCAP,PC-3,DU145)and prostatic epithelial cells(RWPE-1)were compared by Western blotting.PC-3 cells were divided into two groups:SIRT6 transfection group(transfected by SIRT6 overexpression plasmid)and control group(transfected by empty plasmid).Effects of SIRT6 overexpression on proliferation and apoptosis of prostate cancer cells were observed by CCK-8 method and Annexin V and PI double staining.Results:Compared to prostatic epithelial cells,the expressions of SIRT6 in 3 prostate cancer cells were significantly decreased(P<0.01).After transfected,the expression of SIRT6 in PC-3 cells was increased(P<0.01),while the proliferation capacity of PC-3 cells was decreased(P<0.05)and the level of apoptosis was increased(P<0.01).Conclusion:SIRT6 may inhibit the proliferation of prostate cancer cells and promote the apoptosis of prostate cancer cells.

关 键 词：SIRT6 前列腺癌细胞 增殖能力 凋亡 

分 类 号：R285.5[医药卫生—中药学]