苗药熏蒸治疗膝骨关节炎模型兔可延缓细胞外基质的破坏  

Miao medicine fumigation can delay extracellular matrix destruction in a rabbit model of knee osteoarthritis

作　　者：王宝娟 郑曙光 张琪 李田洋 Wang Baojuan;Zheng Shuguang;Zhang Qi;Li Tianyang(The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine,Guiyang 550000,Guizhou Province,China;Guizhou University of Traditional Chinese Medicine,Guiyang 550000,Guizhou Province,China)

机构地区：[1]贵州中医药大学第一附属医院,贵州省贵阳市550000 [2]贵州中医药大学,贵州省贵阳市550000

出　　处：《中国组织工程研究》2022年第8期1180-1186,共7页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(81660832,81960914),项目负责人:郑曙光。

摘　　要：背景:前期研究显示苗药熏蒸疗法对治疗骨性关节炎有确切疗效,但其作用机制不清楚。由于滑膜组织及软骨下骨中血管过度新生是骨性关节炎发生发展的重要原因,血管内皮生长因子是体内重要的促血管新生的因子,其过度表达与基质细胞衍生因子1/CXCR4信号通路存在密切联系。目的:以苗医熏蒸疗法作为干预因素,探讨基质细胞衍生因子1/CXCR4信号通路在骨性关节炎发生发展中的作用及苗药熏蒸疗法的治疗作用机制。方法:健康新西兰大白兔50只,随机分为正常组、模型组、阳性对照组(扶他林软膏外用)、早期干预组、苗药熏蒸组5组,后4组采用膝关节腔注射碘乙酸钠方法建立兔膝骨性关节炎模型,2周后造模成功开始干预治疗,连续治疗6周后取材,苏木精-伊红染色观察滑膜、软骨病理变化;免疫组化方法检测滑膜组织白细胞介素1β、肿瘤坏死因子α水平;Elisa法检测关节液中基质细胞衍生因子1及血清中血管内皮生长因子水平;实时荧光定量PCR检测关节软骨组织中血管内皮生长因子mRNA表达;Western blot检测软骨组织中Ⅱ型胶原蛋白、聚集蛋白聚糖蛋白表达。结果与结论:①与模型组比较:苗药熏蒸组滑膜及软骨组织的炎症表达被显著抑制,病理改变减轻;苗药熏蒸组滑膜白细胞介素1β及肿瘤坏死因子α的积分吸光度值、血清中血管内皮生长因子及关节液中基质细胞衍生因子1水平均降低(P<0.05);苗药熏蒸组软骨血管内皮生长因子mRNA表达降低(P<0.05);软骨中Ⅱ型胶原蛋白、聚集蛋白聚糖表达均升高(P<0.05);②苗药熏蒸组上述指标与阳性对照组及早期干预组比较无显著差异(P>0.05)。实验结果表明:苗药熏蒸疗法可能是通过减少基质细胞衍生因子1与其受体CXCR4结合,抑制白细胞介素1β、肿瘤坏死因子α、血管内皮生长因子表达,延缓细胞外基质破坏,起到治疗骨性关节炎作用。BACKGROUND:Previous studies have shown that Miao medicine fumigation therapy has a definite effect on the treatment of osteoarthritis;however,its mechanism is unclear.Because excessive angiogenesis in synovial tissue and subchondral bone tissue is an important cause of the occurrence and development of osteoarthritis,vascular endothelial growth factor is an important pro-angiogenesis factor in the body,and the stromal cell-derived factor 1/CXCR4 signaling pathway is closely related to the overexpression of vascular endothelial growth factor.OBJECTIVE:To explore the role of stromal cell-derived factor 1/CXCR4 signaling pathway in the osteoarthritic occurrence and development and the therapeutic mechanism of Miao medicine fumigation on knee osteoarthritis.METHODS:Fifty healthy New Zealand white rabbit were randomized into normal,model,positive control(voltaren ointment for external use),early intervention,and Miao medicine fumigation groups.The knee osteoarthritis model was established in the latter four groups by injecting sodium iodoacetate into the knee joint cavity of rabbits.Hematoxylin-eosin staining was used to observe the pathological changes of synovium and cartilage.Immunohistochemical method was used to detect the levels of interleukin-1β and tumor necrosis factor-αin synovial tissue.ELISA method was used to detect the level of stromal cell-derived factor 1 in synovial fluid and the level of vascular endothelial growth factor in serum.Real-time fluorescence quantitative PCR was used to detect vascular endothelial growth factor mRNA expression in articular cartilage tissue.Western blot assay was used to detect the expression of type II collagen and aggrecan protein in cartilage tissue.RESULTS AND CONCLUSION:Compared with the model group,Miao medicine fumigation treatment significantly inhibited the inflammation in synovium and cartilage tissue and reduced its pathological changes.Compared with the model group,the integral absorbance values of interleukin-1βand tumor necrosis factor-αin synovium and th

关 键 词：骨性关节炎 苗药熏蒸 血管内皮生长因子 SDF-1/CXCR4信号通路 基质细胞衍生因子1 兔 动物模型 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学]