海南地区汉、黎民族父源性HLA-G基因14 bp缺失多态性及与重度PE的关系  

Association of 14 bp insertion/deletion polymorphism of HLA-G gene in father with severe PE in Hainan Han and Li population

作　　者：黄丽莉[1] 夏可辉 唐荣[3] 郑林媚[4] HUANG Li-Li;XIA Ke-Hui;TANG Rong;ZHENG Lin-Mei(Department of Obstetrics of Haikou Maternity and Childcare Hospital,Haikou 570203,China)

机构地区：[1]海口市妇幼保健院产科,海口570203 [2]海口市妇幼保健院检验科,海口570203 [3]海南省人民医院肝胆外科,海口570311 [4]海南省人民医院,海南医学院附属海南医院产科,海口570311

出　　处：《中国免疫学杂志》2021年第20期2511-2515,2519,共6页Chinese Journal of Immunology

摘　　要：目的:探讨父源性HLA-G基因第8外显子14 bp缺失多态性与海南汉、黎族重度子痫前期(PE)的相关性及种族间差异。方法:采用PCR和直接测序法对对照组(100对汉族、80对黎族正常妊娠者父儿)、PE组(84对汉族、74对黎族重度子痫患者父儿)HLA-G基因第8外显子14 bp多态性进行分析,比较组间基因型和等位基因频率差异,通过父/儿基因型配伍,比较汉、黎族重度PE组和对照组间基因型配伍频率分布差异。结果:汉族父代、子代HLA-G 14 bp插入/缺失的等位基因及基因型分布在重度PE组和正常妊娠组差异无统计学意义(P>0.05);黎族重度PE组新生儿HLA-G−14 bp频率和−14 bp/−14 bp基因型频率显著低于黎族正常对照组(P=0.003,P=0.001);黎族重度PE组父(−14 bp/−14 bp)/儿(−14 bp/−14 bp)基因型配伍频率显著低于对照组(P=0.003)。结论:HLA-G基因14 bp缺失多态性与汉族重度PE无显著相关性,黎族父(−14 bp/−14 bp)/儿(−14 bp/−14 bp)基因型配伍可能降低母亲发生重度PE的风险。Objective:To investigate correlations between patrilineal HLA-G exon 814 bp deletion polymorphism and severe preeclampsia(PE)in Han and Li ethnic groups in Hainan and its interracial difference.Methods:PCR and direct sequencing methods were used to analyze genotype and allele frequencies of HLA-G exon 814 bp polymorphism in normal control group(100 Han,80 Li)and PE group(84 Han,74 Li),and compared distributions of paternal/fetal genotype case match to analyze differences of HLA-G 14 bp deletion between severe PE and control groups in Han and Li nationalities.Results:There were no differences in allele and genotype frequencies between severe PE and control groups in Han population(P>0.05).Fetal frequencies of HLA-G−14 bp and−14 bp/−14 bp genotype in PE groups were significantly lower than those in control groups in Li population(P=0.003,P=0.001).Fre-quency of father(−14 bp/−14 bp)/fetal(−14 bp/−14 bp)was significantly lower in preeclampsia patients compared with normal con-trols in Li ethnic group(P=0.003).Conclusion:−14 bp deletion polymorphism might have no association with occurrence of PE in Han people of Hainan.Father(−14 bp/−14 bp)/fetal(−14 bp/−14 bp)genotype might reduce risk of early onset severe PE.

关 键 词：HLA-G基因 重度子痫前期 免疫应答 多态性 

分 类 号：R714.24[医药卫生—妇产科学]