单克隆抗体制备技术的最新进展  

Recent advances on preparation technology of monoclonal antibody

作　　者：林金香 王心睿 LIN Jin-Xiang;WANG Xin-Rui(Fujian Children's Hospital,Fuzhou 350011,China)

机构地区：[1]福建省儿童医院,福州350011 [2]福建医科大学附属福建省妇幼保健院医学研究中心,福州350001 [3]国家卫健委非人灵长类生育调节技术评价重点实验室,福州350013

出　　处：《中国免疫学杂志》2021年第20期2544-2548,共5页Chinese Journal of Immunology

基　　金：福建省妇幼保健院科研基金项目(YCXB18-05,YCXM19-03)。

摘　　要：随着各类新兴技术和学科的发展,单克隆抗体制备技术也得到不断更新和完善,但如何利用并有效制备抗体,用于检测、预防、治疗等迫在眉睫。本文综述了单克隆抗体制备技术的最新研究进展,包括抗体库技术、转基因和新型动物模型技术、单个B细胞抗体技术、多学科和计算机辅助抗体制备技术,并简要讨论其应用前景和存在的问题。With the development of a variety of new technologies and disciplines,preparation technology of monoclonal anti-body has been constantly updated and improved,but the use and effective preparation of antibody for disease detection,prevention and treatment is imminent.This review summarizes the latest development of preparation technology of monoclonal antibody,including antibody library technology,transgenic and new animal model technology,single B cell antibody technology,multidisciplinary and computer-aided antibody preparation technology,and briefly discusses its future applications and the existing problems.

关 键 词：单克隆抗体 抗体库 转基因动物 单个B细胞抗体技术 

分 类 号：R392.11[医药卫生—免疫学]