植物源性碱性成纤维细胞生长因子维持人胚胎干细胞的生长与分化  

Plant-derived basic fibroblast growth factor maintains the growth and differentiation of human embryonic stem cells

作　　者：刘峰 彭宇环 罗良平[1] 吴本清 Liu Feng;Peng Yuhuan;Luo Liangping;Wu Benqing(Department of Medical Imaging Center,First Affiliated Hospital,Jinan University,Guangzhou 510630,Guangdong Province,China;Department of Endocrinology,Shenzhen Hospital,University of Chinese Academy of Sciences,Shenzhen 518106,Guangdong Province,China;Department of Pharmacy,Shenzhen Hospital,University of Chinese Academy of Sciences,Shenzhen 518106,Guangdong Province,China;Department of Neonatology,Shenzhen Hospital,University of Chinese Academy of Sciences,Shenzhen 518106,Guangdong Province,China)

机构地区：[1]暨南大学附属第一医院医学影像中心,广东省广州市510630 [2]中国科学院大学深圳医院(光明)内分泌科,广东省深圳市518106 [3]中国科学院大学深圳医院(光明)药学部,广东省深圳市518106 [4]中国科学院大学深圳医院(光明)新生儿科,广东省深圳市518106

出　　处：《中国组织工程研究》2022年第7期1032-1037,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:碱性成纤维细胞生长因子是维持人胚胎干细胞长期培养的重要生长因子,提供来源可靠、无动物源性以及低成本的碱性成纤维细胞生长因子产品对维持人胚胎干细胞生长以及规模化制备至关重要。目的:为了排除动物源性或者其他细菌类的潜在干扰,该研究评估植物源性碱性成纤维细胞生长因子对人胚胎干细胞生长与分化的影响。方法:以人胚胎干细胞系(H9)作为研究材料,采用免疫荧光染色、流式细胞仪检测、碱性磷酸酶染色、RT-PCR方法比较不同质量浓度植物源性碱性成纤维细胞生长因子维持干细胞特性的能力以及其向外、中、内三胚层分化和胰腺细胞分化的潜能。结果与结论:植物源性碱性成纤维细胞生长因子(4μg/L和0.4μg/L)和常规碱性成纤维细胞生长因子(4μg/L)都能很好地维持人胚胎干细胞的生长和分化能力,提示植物源性碱性成纤维细胞生长因子可以作为有效支持人胚胎干细胞生长和分化的备选产品。BACKGROUND:Basic fibroblast growth factor is an important growth factor to maintain the long-term culture of human embryonic stem cells.Providing reliable,non-animal-derived and low-cost basic fibroblast growth factor products is essential for maintaining the growth of human embryonic stem cells and large-scale production.OBJECTIVE:To eliminate the potential interference of animal or other bacteria,this study mainly evaluated the effects of plant-derived basic fibroblast growth factor on the growth and differentiation of human embryonic stem cells.METHODS:Human embryonic stem cells line(H9)was used as research materials.Immunofluorescence staining,flow cytometry detection,alkaline phosphatase staining,and RT-PCR were utilized to systematically compare the ability of plant-derived basic fibroblast growth factor to maintain the characteristics of stem cells at different concentrations and its potential to differentiate into ectoderm,mesoderm,endoderm and pancreatic cells.RESULTS AND CONCLUSION:Plant-derived basic fibroblast growth factor(4μg/L and 0.4μg/L)and basic fibroblast growth factor(4μg/L)used in conventional culture system could maintain the growth and differentiation of human embryonic stem cells,indicating that plant-derived basic fibroblast growth factor can be used as an effective alternative product to support the growth and differentiation of human embryonic stem cells.

关 键 词：干细胞 人胚胎干细胞 碱性成纤维细胞生长因子 植物源性 分化 胰腺细胞 

分 类 号：R459.9[医药卫生—治疗学] R394.2[医药卫生—临床医学]