五脏温阳化瘀汤含药血清干预阿尔茨海默症细胞模型磷酸化tau蛋白的变化  

Effects of serum containing Wuzang Wenyang Huayu Decoction on phosphorylated-tau protein expression in Alzheimer’s disease cell model

作　　者：温小雨 孙玉浩 夏猛[1] Wen Xiaoyu;Sun Yuhao;Xia Meng(Guangxi University of Traditional Chinese Medicine,Nanning 530000,Guangxi Zhuang Autonomous Region,China)

机构地区：[1]广西中医药大学,广西壮族自治区南宁市530000

出　　处：《中国组织工程研究》2022年第7期1068-1073,共6页Chinese Journal of Tissue Engineering Research

基　　金：广西中医药大学第一批“歧黄工程”高层次人才团队培育项目(2018001),项目负责人:夏猛;国家自然科学基金地区基金项目(81860805),项目负责人:夏猛;中医基础研究重点实验室系统课题(KJT14004,KJT1701202),项目负责人:夏猛。

摘　　要：背景:前期各项研究表明,五脏温阳化瘀汤对阿尔茨海默症具有良好的治疗效果,但其药理机制尚未完全阐明。目的:探讨五脏温阳化瘀汤含药血清对阿尔茨海默症细胞模型过氧化物酶体增殖物激活受体γ和磷酸化tau蛋白表达的影响。方法:通过β-淀粉样蛋白干预原代海马神经元细胞建立阿尔茨海默症细胞模型,给予五脏温阳化瘀汤含药血清、盐酸多奈哌齐含药血清干预72 h,采用免疫荧光法检测原代神经细胞树突长度及分支数,Western blot检测过氧化物酶体增殖物激活受体γ和磷酸化tau蛋白表达。结果与结论:与空白对照组比较,模型组神经元细胞树突长度及分支数明显下降(P<0.05),过氧化物酶体增殖物激活受体γ蛋白表达下降,磷酸化tau蛋白表达升高(P<0.05或P<0.01);与模型组比较,五脏温阳化瘀汤组与盐酸多奈哌齐组神经元树突长度及分支数增加,过氧化物酶体增殖物激活受体γ蛋白表达升高,磷酸化tau蛋白表达显著下降(P<0.05或P<0.01)。结果表明,五脏温阳化瘀汤对β-淀粉样蛋白干预的阿尔茨海默症细胞模型具有神经保护作用,其机制可能与上调过氧化物酶体增殖物激活受体γ蛋白表达,抑制Tau蛋白的磷酸化相关。BACKGROUND:Various previous studies have shown that Wuzang Wenyang Huayu Decoction has a good therapeutic effect on Alzheimer’s disease,but its pharmacological mechanism has not been fully elucidated.OBJECTIVE:To investigate the effect of Wuzang Wenyang Huayu Decoction drug-containing serum on the expression of peroxisome proliferator-activated receptor γ and phosphorylated-tau protein in Alzheimer’s disease cell model.METHODS:Beta amyloid protein was used to induce primary hippocampal neurons to establish the currently recognized Alzheimer’s disease cell model.Wuzang Wenyang Huayu Decoction drug-containing serum and donepezil hydrochloride drug-containing serum were given for intervention for 72 hours.The dendritic length and branch number of primary neurons cells were detected by immunofluorescence assay.The expression levels of peroxisome proliferator-activated receptor γ and phosphorylated-tau protein were detected by western blot assay.RESULTS AND CONCLUSION:Compared with the control group,the length and branch number of dendritic cells in the model group were significantly decreased(P<0.05),peroxisome proliferator-activated receptorγ expression decreased,and phosphorylated-tau protein increased(P<0.05 or P<0.01).Compared with the model group,the dendritic length and branch number of neurons in Wuzang Wenyang Huayu group and donepezil hydrochloride group increased,the expression of PPARγ increased,and the expression of phosphorylated-tau protein significantly decreased(P<0.05 or P<0.01).Results confirmed that Wuzang Wenyang Huayu Decoction has neuroprotective effect on Alzheimer’s disease cells induced by beta amyloid protein,and its mechanism may be related to upregulation of peroxisome proliferator-activated receptorγprotein and inhibition of phosphorylated-tau protein.

关 键 词：神经元 阿尔茨海默症 五脏温阳化瘀汤 β-淀粉样蛋白 过氧化物酶体增殖物激活受体Γ 磷酸化TAU蛋白 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学]