髓芯减压联合牙髓干细胞治疗兔早期激素性股骨头坏死  

Core decompression combined with dental pulp stem cells in the treatment of steroid-associated femoral head necrosis in rabbits

作　　者：王新民[1] 刘飞[1] 许杰[1] 白玉玺 吕剑 Wang Xinmin;Liu Fei;Xu Jie;Bai Yuxi;LüJian(Ward Two,Department of Orthopedics,First Hospital of Qinhuangdao,Qinhuangdao 066000,Hebei Province,China)

机构地区：[1]秦皇岛市第一医院骨科二病区,河北省秦皇岛市066000

出　　处：《中国组织工程研究》2022年第7期1074-1079,共6页Chinese Journal of Tissue Engineering Research

基　　金：2019年度中央引导地方科技发展专项资金项目(20190214175848679),项目负责人:刘飞。

摘　　要：背景:牙髓干细胞具有强大的增殖能力和多向分化潜能,在骨和软骨组织工程中的应用取得了明显进展,因此研究牙髓干细胞对股骨头坏死的治疗作用,将为股骨头坏死患者带来新的治疗策略和希望。目的:观察髓芯减压联合牙髓干细胞对兔早期激素型股骨头坏死的治疗作用。方法:成年健康新西兰大白兔52只,随机分为正常对照组、模型组、溶媒组、牙髓干细胞组,除正常对照组外,其余3组采用激素型股骨头坏死造模方法。造模成功后(第4周),溶媒组和牙髓干细胞组右侧行髓芯减压术,牙髓干细胞组于股骨头减压孔注射牙髓干细胞,溶媒组同时注射同体积生理盐水,第4,5,6周共注射3次。第12周检测骨密度、骨形态学参数、空骨陷窝率、骨小梁面积比等指标。结果与结论:①影像学检查提示溶媒组和牙髓干细胞组治疗效果均较模型组有显著改善;与溶媒组比较,牙髓干细胞组骨体积分数、骨小梁数量、骨密度改善更明显,差异有显著性意义(P<0.05);②牙髓干细胞组空骨陷窝率和骨小梁面积比与正常对照组更为接近,溶媒组空骨陷窝率高于正常对照组(P<0.05),骨小梁面积比低于正常对照组(P<0.05);溶媒组和牙髓干细胞组空骨陷窝率明显低于模型组(P<0.05),骨小梁面积比明显高于模型组(P<0.05);③结果表明,牙髓干细胞联合髓芯减压治疗早期股骨头坏死优于单纯髓芯减压。BACKGROUND:The dental pulp stem cells have strong proliferation ability and multi differentiation potential ability,which plays an important role in bone and cartilage tissue engineering.Therefore,to study the therapeutic effect of dental pulp stem cells on femoral head necrosis will bring new treatment strategies and hope for patients with femoral head necrosis.OBJECTIVE:To observe the therapeutic effect of core decompression combined with dental pulp stem cells on early steroid-associated femoral head necrosis in rabbits.METHODS:Fifty-two adult healthy New Zealand white rabbits were randomly divided into four groups:normal control group,model group,solvent group,and dental pulp stem cell group.Except the normal control group,the model of steroid induced osteonecrosis of femoral head was established in the other three groups.After successful modeling(the 4^(th) week),core decompression was performed on the right side in the solvent group and dental pulp stem cell group.The dental pulp stem cell group was injected with dental pulp stem cells in the decompression hole of the femoral head,and the solvent group was injected with the same volume of sodium chloride injection at the same time.There were three injections in the 4^(th),5^(th) and 6^(th) weeks.At the 12^(th) week,indicators such as bone density,bone morphology parameters,empty bone lacuna rate,and bone trabecular area ratio were measured.RESULTS AND CONCLUSION:(1)Imaging examination showed that the treatment effects of solvent group and dental pulp stem cell group were significantly improved compared with the model group.Bone volume fraction,trabecular number,and bone mineral density of femoral head in the dental pulp stem cell group were better than those in the solvent group,and the difference was statistically significant(P<0.05).(2)Compared with the normal control group,the empty bone lacuna rate and trabecular area ratio of the dental pulp stem cell group were closer;the empty bone lacuna rate of the solvent group was higher than that of the normal c

关 键 词：干细胞 牙髓干细胞 髓芯减压 激素型股骨头坏死 兔 病理 动物模型 

分 类 号：R459[医药卫生—治疗学] R318[医药卫生—临床医学]