干细胞源外泌体促进糖尿病创面愈合的机制及应用  

Mechanism and application of stem cell-derived exosomes in promoting diabetic wound healing

作　　者：张璟琳 冷敏 朱博恒 汪虹[1] Zhang Jinglin;Leng Min;Zhu Boheng;Wang Hong(Department of Burns,the Second Affiliated Hospital,Kunming Medical University,Burn Institute of Yunnan Province,Kunming 650101,Yunnan Province,China)

机构地区：[1]昆明医科大学第二附属医院烧伤科,云南省烧伤研究所,云南省昆明市650101

出　　处：《中国组织工程研究》2022年第7期1113-1118,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金地区科学基金项目(81660321),项目负责人:汪虹;云南省科技厅-昆明医科大学应用基础研究联合专项资助项目[2017FE468(-177)],项目负责人:汪虹。

摘　　要：背景:外泌体是成体干细胞旁分泌的重要活性成分之一,为干细胞“非细胞”治疗提供了基础,干细胞来源外泌体可通过不同机制促进糖尿病创面愈合。目的:总结干细胞源外泌体在治疗糖尿病创面中的主要机制及其应用途径。方法:用计算机检索PubMed数据库、Web of Science数据库、FMRS外文医学信息资源检索平台等英文数据库及中国知网、万方数据知识服务平台等中文数据库,检索范围从建库至2020年9月发表的文献,英文检索词为“stem cells,exosomes,wound healing,cell proliferation,neovascularization,inflammation,extracellular matrix,therapeutic use”,中文检索词为“干细胞,外泌体,伤口愈合,细胞增殖,血管新生,炎症,细胞外基质,治疗应用”。根据纳入和排除标准最终纳入66篇文献进行结果分析。结果与结论:①总结了干细胞源外泌体通过调节炎症、促进血管新生、再上皮化和细胞外基质重塑等方面促进糖尿病伤口愈合;②列举了干细胞源外泌体在糖尿病创面中的应用途径;③干细胞源外泌体能避免干细胞治疗糖尿病创面的免疫性反应,并且其对糖尿病创面的调控范围更广、效果更优;④目前干细胞源外泌体在糖尿病创面中的应用还存在一些问题,例如:干细胞源外泌体分离纯度低、分离成本高、生产效率低、到达目的区域的途径以及损耗率、扩散效率等。BACKGROUND:The exosomes are one of important active components of adult stem cells paracrine,providing the basis for stem cell“non-cellular”treatment.Exosomes from stem cells can play different roles in the wound through different mechanisms to promote the healing of diabetic wounds.OBJECTIVE:To summarize the main mechanism and application of stem cell derived exosomes in the treatment of diabetic wounds.METHODS:Computers were used to retrieve English databases such as PubMed database,Web of Science database,FMRS foreign language medical information resource retrieval platform,and Chinese databases such as CNKI and Wanfang Data Knowledge Service Platform.The retrieval scope was from the inception of the database to September 2020.The English and Chinese search terms were“stem cells,exosomes,wound healing,cell proliferation,neovascularization,inflammation,extracellular matrix,therapeutic use”.According to the inclusion and exclusion criteria,66 articles were included and the results were analyzed.RESULTS AND CONCLUSION:(1)It is summarized that stem cell-derived exosomes can promote diabetic wound healing by regulating inflammation,promoting neovascularization,re-epithelialization and remodeling of extracellular matrix.(2)The application of stem cell-derived exosomes in diabetic wounds was listed.(3)The exosomes from stem cells can avoid the immune response of stem cells in the treatment of diabetic wounds,and the genetic material of stem cells carried by them is more widely regulated and more effective than that from ordinary cells in the treatment of diabetic wounds.(4)At present,the application of stem cell derived exosomes in diabetes wounds still has some problems,such as low purity,high cost of separation,low production efficiency,and the way to reach the target area,loss rate and diffusion efficiency.

关 键 词：干细胞 外泌体 糖尿病创面 细胞增殖 血管生成 创面愈合 组织工程 综述 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学]