电活性生物膜促进大鼠的体内成骨  

Electroactive membrane promotes bone formation in rats in vivo

作　　者：何逸恒 程鸣威 朱培君 许言 陈家豪 赖春花 徐淑兰 He Yiheng;Cheng Mingwei;Zhu Peijun;Xu Yan;Chen Jiahao;Lai Chunhua;Xu Shulan(Stomatological Hospital of Southern Medical University,Guangzhou 510000,Guangdong Province,China)

机构地区：[1]南方医科大学口腔医院,广东省广州市510000

出　　处：《中国组织工程研究》2022年第28期4446-4451,共6页Chinese Journal of Tissue Engineering Research

基　　金：广东省中医药局科研项目(20211274),项目负责人:徐淑兰;南方医科大学口腔医院科研培育计划项目(PY2020011),项目负责人:徐淑兰。

摘　　要：背景:课题组前期研究发现,极化聚偏氟乙烯-三氟乙烯生物膜体外具有调控成骨细胞增殖分化的作用,然而其体内促进骨缺损愈合的作用尚未明确。目的:建立大鼠下颌骨临界骨缺损的动物模型,评价极化聚偏氟乙烯-三氟乙烯在体内促进骨缺损愈合的作用。方法:采用流延法制备聚偏氟乙烯-三氟乙烯生物膜,经由电晕极化处理后使其获得良好且稳定的压电性能,表征极化前后生物膜的物理化学性能、电学性能与生物相容性。在18只Wistar大鼠双侧下颌角制备直径4 mm的临界全厚骨缺损,随机分为3组(n=6),实验组植入极化生物膜,对照组植入未极化生物膜,空白对照组不做处理,术后4,8周取材,采用Micro-CT扫描分析各组的骨形成量,采用组织染色观察各组骨形成情况。实验方案经南方医科大学动物实验伦理委员会批准,伦理批件号:第00225515号。结果与结论:①聚偏氟乙烯-三氟乙烯生物膜极化后的表面形貌、水接触角、弹性模量及最大拉伸强度无明显变化,电学常数为-10 pc/N,表面电势为-83 mV;②细胞增殖-毒性检测结果显示,极化生物膜促进了骨髓间充质干细胞的增殖,未对细胞产生毒性反应;③术后4,8周的Micro-CT结果显示,实验组骨缺损区骨形成量显著高于对照组、空白对照组(P<0.05);④术后4,8周的苏木精-伊红染色显示,实验组骨组织愈合速度与质量明显优于对照组、空白对照组;⑤术后4,8周的免疫组化染色显示,实验组骨缺损区的Runx2表达高于对照组、空白对照组(P<0.05);⑥结果表明,极化生物膜具有良好的理化性能、生物相容性与体内促成骨性能。BACKGROUND:The previous research of our group found that polarized poly(vinylidenefluoride-trifluoroethylene)has the effect on regulating the proliferation and differentiation of osteoblasts in vitro,but the role of polarized poly(vinylidenefluoride-trifluoroethylene)membrane in promoting bone defect healing in vivo is not clear.OBJECTIVE:To establish a rat model of critical bone defect in mandible and evaluate the effect of polarized poly(vinylidenefluoride-trifluoroethylene)in promoting the healing of bone defect in vivo.METHODS:The poly(vinylidenefluoride-trifluoroethylene)membrane was prepared by flow-casting method,and after corona polarization treatment,it obtained good and stable piezoelectric properties.The physicochemical properties,electrical properties and biocompatibility of the membrane before and after polarization were characterized.Critical full-thickness bone defects with a diameter of 4 mm were prepared in eighteen Wistar rats with bilateral mandibular angles.Rats were randomly divided into three groups(n=6).Polarized poly(vinylidenefluoride-trifluoroethylene)membrane was implanted in the experimental group,and unpolarized poly(vinylidenefluoride-trifluoroethylene)membrane was implanted in the control group.The blank control group was not treated.At 4 and 8 weeks,Micro-CT scanning was used to analyze the amount of bone formation in each group,and tissue staining was used to observe the bone formation in each group.The experimental protocol was approved by the Animal Experiment Ethics Committee of Southern Medical University(approval No.00225515).RESULTS AND CONCLUSION:(1)The surface morphology,water contact angle,elastic modulus and maximum tensile strength of poly(vinylidenefluoridetrifluoroethylene)membrane after polarization had no obvious changes.The electrical constant was-10 pc/N,and the surface potential was-83 mV.(2)The results of cell proliferation-toxicity assay showed that the polarized poly(vinylidenefluoride-trifluoroethylene)membrane promoted the proliferation of bone marrow mesenc

关 键 词：仿生电活性 压电聚合物 电活性材料 引导骨再生 引导骨再生膜 骨组织工程 骨替代材料 临界骨缺损 

分 类 号：R459.9[医药卫生—治疗学] R318.5[医药卫生—临床医学]