壳聚糖/海藻酸盐复合支架联合山楂叶总黄酮修复脊髓损伤  

Chitosan/alginate composite scaffold combined with hawthorn leaf flavonoids for spinal cord injury repair

作　　者：刘名[1] 王凯[1] Liu Ming;Wang Kai(First Department of Orthopedics,Qinghai Provincial People’s Hospital,Xining 810000,Qinghai Province,China)

机构地区：[1]青海省人民医院骨科一病区,青海省西宁市810000

出　　处：《中国组织工程研究》2022年第28期4466-4471,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:有研究证明山楂叶总黄酮对脊髓损伤具有一定的治疗作用,但是给药方式局限于腹腔注射,而直接应用于脊髓损伤部位的报道较少见。目的:比较负载山楂叶总黄酮缓释微球壳聚糖海藻酸盐复合支架与腹腔注射山楂叶总黄酮联合壳聚糖海藻酸盐复合支架修复脊髓损伤的效果。方法:制备壳聚糖海藻酸盐复合支架、山楂叶总黄酮缓释微球与负载山楂叶总黄酮缓释微球的壳聚糖海藻酸盐复合支架。取40只SD大鼠,建立脊髓全切损伤模型,随机分4组:损伤对照组术后腹腔注射生理盐水;支架组植入壳聚糖海藻酸盐复合支架,术后腹腔注射生理盐水;支架+药物注射组植入壳聚糖海藻酸盐复合支架,术后腹腔注射20 mg/(kg·d)的山楂叶总黄酮;负载药物支架组植入负载缓释微球的壳聚糖海藻酸盐复合支架,术后腹腔注射生理盐水。术后8周内应用BBB评分与斜板实验评估大鼠运动功能,术后8周时进行脊髓组织病理组织形态观察及Western Blot检测。结果与结论:①自术后2周开始,植入支架3组大鼠的BBB评分与斜板实验角度均高于损伤对照组(P<0.05),支架+药物注射组、负载药物支架组高于支架组(P<0.05),负载药物支架组高于支架+药物注射组(P<0.05);②苏木精-伊红染色显示,损伤对照组损伤区域未见脊髓组织,其余3组植入的支架与损伤脊髓端连接较紧密,并且支架材料中长入了组织与细胞,支架+药物注射组与负载药物支架组长入的组织较多;③Western Blot检测显示,植入支架3组的胶质纤维酸性蛋白表达低于损伤对照组(P<0.05),神经丝蛋白200、髓磷脂碱性蛋白、生长相关蛋白43表达高于损伤对照组(P<0.05);负载药物支架组的胶质纤维酸性蛋白表达低于支架组、支架+药物注射组(P<0.05),支架+药物注射组低于支架组(P<0.05);负载药物支架组的神经丝蛋白200、髓磷脂碱性蛋白、生长相关蛋白43表达高�BACKGROUND:Studies have shown that hawthorn leaf total flavonoids have a certain therapeutic effect on spinal cord injury,but the method of administration is limited to intraperitoneal injection,and there are rare reports of direct application to the site of spinal cord injury.OBJECTIVE:To compare the effects of chitosan alginate composite scaffold loaded with hawthorn leaf flavonoids sustained-release microspheres and intraperitoneal injection of hawthorn leaf flavonoids combined with chitosan alginate composite scaffold in the repair of spinal cord injury.METHODS:Chitosan alginate composite scaffolds,hawthorn leaf flavonoids sustained-release microspheres and chitosan alginate composite scaffolds loaded with hawthorn leaf total flavonoids sustained-release microspheres were prepared.Forty Sprague-Dawley rats were used to establish a model of complete spinal cord injury and were randomly divided into four groups.Injury control group received intraperitoneal injection of saline after surgery.Rats in the stent group were implanted with a chitosan alginate composite stent,and injected intraperitoneally with physiological saline after the operation.Rats in the stent+drug injection group were implanted with chitosan alginate composite stent,and injected intraperitoneally with 20 mg/(kg·d)hawthorn leaf total flavonoids after surgery.Rats in the drug loaded stent group were implanted with chitosan alginate composite stent loaded with sustained-release microspheres,and injected intraperitoneally with physiological saline after the operation.BBB score and inclined plate test were used to evaluate the motor function of rats within 8 weeks after operation.The pathological morphology and western blot assay of spinal cord tissue were observed at 8 weeks after operation.RESULTS AND CONCLUSION:(1)Since 2 weeks after operation,BBB score and inclined plate test angle of three groups of stent implantation were higher than those of injury control group(P<0.05),and those of stent+drug injection group and drug loaded stent group we

关 键 词：壳聚糖 海藻酸盐 山楂叶总黄酮 脊髓损伤 复合支架 缓释微球 

分 类 号：R459.9[医药卫生—治疗学] R318.08[医药卫生—临床医学]