牙种植体周围骨组织修复中microRNA的作用及调控机制  

Role and regulatory mechanism of microRNA in bone tissue repair around dental implants

作　　者：杨琼琼 李萍 王倩 Yang Qiongqiong;Li Ping;Wang Qian(Department of Prosthodontics,Affiliated Stomatological Hospital of Zunyi Medical University,Zunyi 563000,Guizhou Province,China;Department of Pathology,Affiliated Stomatological Hospital of Zunyi Medical University,Zunyi 563000,Guizhou Province,China;Oral Disease Research Key Laboratory of Guizhou Tertiary Institution,School of Stomatology,Zunyi Medical University,Zunyi 563000,Guizhou Province,China)

机构地区：[1]遵义医科大学附属口腔医院修复科,贵州省遵义市563000 [2]遵义医科大学附属口腔医院病理科,贵州省遵义市563000 [3]贵州省普通高等学校口腔疾病研究特色重点实验室暨遵义市口腔疾病研究重点实验室,遵义医科大学,贵州省遵义市563000

出　　处：《中国组织工程研究》2022年第28期4539-4545,共7页Chinese Journal of Tissue Engineering Research

摘　　要：背景:细胞功能的调节是骨内种植体表面骨形成的关键,从细胞和分子的角度理解骨重建有助于提高牙种植体植入的成功率。研究发现microRNA(miRNA)作为控制细胞功能的关键因子,在牙种植体周围骨组织的细胞分化调控中可发挥重要作用。目的:文章对miRNAs在牙种植体周围骨组织修复中的作用机制、牙种植体表面形貌对miRNAs表达的影响以及miRNAs与骨组织修复的应用研究进展进行综述。方法:由第一作者在PubMed、Medline、Sciencedirect数据库检索,检索词为“microRNA,bone remodeling,dental implants,mesenchymal stem cells,titanium,osseointegration”,阅读文题、摘要进行初步筛选,排除与文章主题无关的研究及试验,最后纳入61篇文献进行结果分析。结果与结论:①miRNAs通过直接靶向Runx2、Osterix基因或者影响其他增强或抑制Runx2、Osterix的基因表达水平,并与骨形态发生蛋白/Smads、Wnt/β-catenin信号通路和整合素等相互作用来参与成骨细胞分化的过程;②miRNAs通过刺激成骨细胞标记基因的表达调节成骨-血管生成偶联;③多项研究证实钛种植体的表面形貌影响miRNAs的表达,微/纳米结构对成骨细胞的增殖和分化具有更好的促进作用,具有纳米粗糙形貌的表面通过表达与成骨基因相关的miRNAs来改善骨组织修复的反应;④钛种植体微弧氧化涂层通过激活miRNAs促进间充质干细胞成骨分化,将miRNAs与微弧氧化钛植入物复合构建基因修饰的组织工程植入体是一个可行的方法。BACKGROUND:The regulation of cell function is the key to bone formation on the surface of dental implants.Understanding bone reconstruction from the perspective of cells and molecules is helpful to improve the success rate of dental implants.Studies have found that microRNA(miRNA),as a key factor controlling cell function,plays an important role in the regulation of cell differentiation of bone tissue around dental implants.OBJECTIVE:To review the regulation mechanism of miRNA in bone tissue repair around dental implants,the influence of dental implant surface morphology on miRNA expression,and the application of miRNA in bone tissue repair.METHODS:Articles were searched by the first author on PubMed,Medline,and Sciencedirect databases.Search terms were“microRNA,bone remodeling,dental implants,mesenchymal stem cells,titanium,osseointegration”.Preliminary screening was performed by reading the title and abstract.After excluding the research and experiment unrelated to the topic of the article,61 articles were finally included for result analysis.RESULTS AND CONCLUSION:(1)miRNAs participate in osteoblast differentiation by directly targeting Runx2 and Osterix genes or influencing other genes that enhance or inhibit the expression level of Runx2 and Osterix,and interacting with bone morphogenetic protein/Smads,Wnt/β-catenin signaling pathway and integrin.(2)miRNAs regulate osteogenesis-angiogenesis coupling by stimulating the expression of osteoblast marker genes.(3)Many experiments have confirmed that the surface morphology of titanium implants affects the expression of miRNA,and micro/nano structure can promote the proliferation and differentiation of osteoblasts better.The surface with nano rough morphology can improve the response of bone tissue repair by expressing miRNA related to osteogenic genes.(4)Micro-arc oxidation coating of titanium implants can promote the osteogenic differentiation of mesenchymal stem cells by activating miRNAs.It is feasible to construct genetically modified tissue engineering i

关 键 词：骨重建 MICRORNA 牙种植体 种植体表面 钛 成骨细胞 破骨细胞 间充质干细胞 miRNA 

分 类 号：R459.9[医药卫生—治疗学] R363[医药卫生—临床医学]