顺序注射原子荧光光度法测定医院污水中总砷与总汞的正交试验  

Orthogonal test for determination of total arsenic and mercury in hospital sewage by sequential injection atomic fluorescence

作　　者：罗霞[1] 陈杰[1] 伍剑[1] 黄亚婷 吉华贵[1] LUO Xia;CHEN Jie;WU Jian;HUANG Ya-ting;JI Hua-gui(Deyang Municipal Center for Disease Control and Prevention,Deyang,Sichuan 618000,China)

机构地区：[1]四川省德阳市疾病预防控制中心,四川德阳618000

出　　处：《疾病预防控制通报》2021年第5期90-94,共5页Bulletin of Disease Control & Prevention(China)

摘　　要：目的建立顺序注射原子荧光光度计同时测定医院污水中总砷、总汞的方法。方法按照L_(9)(3^(4))正交表,以硼氢化钾浓度、硝酸浓度、硫脲浓度、抗坏血酸浓度为影响因素,每个因素选取3个浓度水平,共设计9组试验来确定最佳实验条件。结果测得9组试验中总砷的荧光强度为91.01~1479.01,总汞的荧光强度524.19~1421.45;影响砷荧光强度的因素中,硝酸的极差最大(1030.42),硼氢化钾的极差最小(28.06);影响汞荧光强度的因素中,硼氢化钾的极差最大(578.49),抗坏血酸的极差最小(59.16)。砷和汞的相对标准偏差(RSD)分别为0.63%和0.76%,加标回收率分别为94%~105%和93%~105%。结论硝酸浓度是影响总砷荧光强度的重要因素,硼氢化钾浓度是影响总汞荧光强度的重要因素。0.5%硼氢化钾、3.0%硝酸、2.0%硫脲和2.0%抗坏血酸为同时测定医院污水中总砷、总汞的最佳实验条件,准确度高、精密度好,能满足医院污水中总砷和总汞的快速批量检测。Objective To establish the method for simultaneous determination of total arsenic and mercury in hospital wastewater by sequential injection atomic fluorescence spectrometry.Methods According to the L_(9)(3^(4))orthogonal table,the concentration of potassium borohydride,nitric acid,thiourea and ascorbic acid were set as the influencing factors.There were 3 concentration levels selected for each factor,and 9 groups of the experiments were designed to determine the optimal experimental conditions.Results The fluorescence intensity was from 91.01 to 1479.01 of the total arsenic,and from524.19 to 1421.45 of the total mercury in the 9 groups of determination.Out of the factors influencing the fluorescence intensity of arsenic,the extreme difference was maximum in nitric acid(1030.42),and minimum in potassium borohydride(28.06).Among the factors influencing the fluorescence intensity of mercury,the extreme difference was the biggest in potassium borohydride(578.49),and the least in ascorbic acid(59.16).The relative standard deviation(RSD)was0.63%of arsenic and 0.76%of mercury,with the rate of adding standard recovery from 94%to 105%and 93%to 105%,respectively.Conclusions The key factor affecting the fluorescence intensity is the concentration of nitric acid in total arsenic,and the concentration of potassium borohydride in total mercury.The potassium borohydride of 0.5%,nitric acid of3.0%,thiourea of 2.0%and ascorbic acid of 2.0%are the optimal experimental conditions for simultaneous determination of total arsenic and total mercury in hospital sewage,with high accuracy and good precision,and suitable for the rapid detection for total arsenic and mercury in batch samples of hospital sewage.

关 键 词：总砷 总汞 原子荧光 正交试验 医院污水 

分 类 号：R123.1[医药卫生—环境卫生学] X799.5[医药卫生—公共卫生与预防医学]